Dalvin Sussie, Anselmo Mark A, Prodhan Parthak, Komatsuzaki Katsumi, Schnitzer Jay J, Kinane T Bernard
Department of Pediatrics, Massachusetts General Hospital for Children, Harvard Medical School, Boston, MA 02114, USA.
Gene Expr Patterns. 2003 Jun;3(3):279-83. doi: 10.1016/s1567-133x(03)00047-4.
The ligand Netrin-1 and its receptors DCC and UNC5H2 are critical for the regulation of neuronal migration in nervous system development. Here we demonstrate expression of these molecules in lung development. The mRNA expression profiles of Netrin-1, DCC and UNC5H2 are developmentally regulated during embryonic mouse lung formation. Netrin-1 shows a bimodal expression pattern with elevated mRNA levels early followed by a second peak in late gestation. Peak expression of DCC occurs early in development whereas expression of UNC5H2 peaks late in development. We also demonstrate localization of Netrin-1, DCC and UNC5H2 during the stages of lung development. We present evidence that these proteins are modulated spatially in the mesenchyme and epithelium during lung organogenesis.
配体Netrin-1及其受体DCC和UNC5H2对神经系统发育过程中神经元迁移的调节至关重要。在此，我们证明了这些分子在肺发育中的表达。Netrin-1、DCC和UNC5H2的mRNA表达谱在胚胎小鼠肺形成过程中受到发育调控。Netrin-1呈现双峰表达模式，早期mRNA水平升高，随后在妊娠后期出现第二个峰值。DCC的表达峰值出现在发育早期，而UNC5H2的表达峰值出现在发育后期。我们还证明了Netrin-1、DCC和UNC5H2在肺发育各阶段的定位。我们提供的证据表明，这些蛋白质在肺器官发生过程中在间充质和上皮中受到空间调节。
