Ruiz M F, Goday C, González P, Sánchez L
Centro de Investigaciones Biológicas, Velázquez 144, 28006 Madrid, Spain.
Gene Expr Patterns. 2003 Jun;3(3):341-6. doi: 10.1016/s1567-133x(03)00031-0.
This paper reports the cloning and characterization in Sciara ocellaris of the gene homologous to Sex-lethal (Sxl) of Drosophila melanogaster. This gene plays the key role controlling sex determination and dosage compensation in the latter species. The Sciara Sxl gene produces a single transcript encoding a single protein in both males and females. This protein, found inside the nucleus, is expressed in all tissues. Both Sciara and Drosophila Sxl proteins are highly conserved at their two RNA-binding domains. In both Sciara sexes, the Sxl protein co-localizes with transcription-active regions dependent on RNA polymerase II but not on RNA polymerase I. It would appear that in Sciara, Sxl does not appear to play the key discriminative role in controlling sex determination and dosage compensation that it plays in Drosophila.
本文报道了在眼蕈蚊中克隆和鉴定与黑腹果蝇性致死基因（Sex-lethal，Sxl）同源的基因。该基因在后者物种中起着控制性别决定和剂量补偿的关键作用。眼蕈蚊的Sxl基因在雄性和雌性中都产生一个编码单一蛋白质的转录本。这种蛋白质存在于细胞核内，在所有组织中都有表达。眼蕈蚊和果蝇的Sxl蛋白在其两个RNA结合结构域高度保守。在眼蕈蚊的两性中，Sxl蛋白与依赖于RNA聚合酶II而非RNA聚合酶I的转录活性区域共定位。似乎在眼蕈蚊中，Sxl在控制性别决定和剂量补偿方面似乎并不发挥其在果蝇中所起的关键判别作用。
