Aitchison J D, Szilard R K, Nuttley W M, Rachubinski R A
Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada.
Yeast. 1992 Sep;8(9):721-34. doi: 10.1002/yea.320080905.
The carboxyl-terminal tripeptide Ala-Lys-Ile is essential for targeting Candida tropicalis trifunctional enzyme (hydratase-dehydrogenase-epimerase) to peroxisomes of both Candida albicans and Saccharomyces cerevisiae (Aitchison,J.D., Murray, W.W. and Rachubinski, R. A. (1991).J. Biol. Chem. 266, 23197-23203). We investigated the possibility that this tripeptide may act as a general peroxisomal targeting signal (PTS) for other proteins in the yeasts C. tropicalis, C. albicans, Yarrowia lipolytica and S. cerevisiae, and in rat liver. Anti-AKI antibodies raised against the carboxyl-terminal 12 amino acids of trifunctional enzyme were used to search for this PTS in proteins of these yeasts and of rat liver. The anti-AKI antibodies reacted exclusively with multiple peroxisomal proteins from the yeasts C. tropicalis, C. albicans and Y. lipolytica. There was a weak reaction of the antibodies with one peroxisomal protein from S. cerevisiae and no reaction with peroxisomal proteins from rat liver. Antibodies directed against a synthetic peptide containing a carboxyl-terminal Ser-Lys-Leu PTS (Gould, S. J., Krisans, S., Keller, G.-A. and Subramani, S. (1990). J. Cell Biol. 110,27-34) reacted with multiple peroxisomal proteins of rat liver and with peroxisomal proteins of yeast distinct from those identified with anti-AKI antibodies. These results provide evidence that several peroxisomal proteins of different yeasts contain a PTS antigenically similar to that of C. tropicalis trifunctional enzyme and that this signal is absent from peroxisomal proteins from at least one mammalian system, rat liver.
羧基末端三肽丙氨酸-赖氨酸-异亮氨酸对于将热带假丝酵母三功能酶（水化酶-脱氢酶-表异构酶）靶向白色念珠菌和酿酒酵母的过氧化物酶体至关重要（艾奇逊，J.D.，默里，W.W.和拉楚宾斯基，R.A.（1991年）。《生物化学杂志》266，23197 - 23203）。我们研究了这种三肽是否可能作为热带假丝酵母、白色念珠菌、解脂耶氏酵母和酿酒酵母以及大鼠肝脏中其他蛋白质的通用过氧化物酶体靶向信号（PTS）。针对三功能酶羧基末端12个氨基酸产生的抗AKI抗体用于在这些酵母和大鼠肝脏的蛋白质中寻找这种PTS。抗AKI抗体仅与来自热带假丝酵母、白色念珠菌和解脂耶氏酵母的多种过氧化物酶体蛋白发生反应。该抗体与来自酿酒酵母的一种过氧化物酶体蛋白反应较弱，与来自大鼠肝脏的过氧化物酶体蛋白无反应。针对含有羧基末端丝氨酸-赖氨酸-亮氨酸PTS的合成肽产生的抗体（古尔德，S.J.，克里桑斯，S.，凯勒，G.-A.和苏布拉马尼，S.（1990年）。《细胞生物学杂志》110，27 - 34）与大鼠肝脏的多种过氧化物酶体蛋白以及与抗AKI抗体鉴定的不同的酵母过氧化物酶体蛋白发生反应。这些结果提供了证据，表明不同酵母的几种过氧化物酶体蛋白含有与热带假丝酵母三功能酶抗原性相似的PTS，并且该信号在至少一种哺乳动物系统大鼠肝脏的过氧化物酶体蛋白中不存在。
