Takade Akemi, Umeda Akiko, Matsuoka Masanori, Yoshida Shin-ichi, Nakamura Masahiro, Amako Kazunobu
Department of Bacteriology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Fukuoka 812-8582, Japan.
Microbiol Immunol. 2003;47(4):265-70. doi: 10.1111/j.1348-0421.2003.tb03394.x.
The cell envelope and cytoplasmic architecture of the Mycobacterium leprae Thai-53 strain were examined using the freeze-substitution technique of electron microscopy and compared with those of the M. tuberculosis H37Rv strain. Both strains had similarly multilayered envelope architectures composed of an electron-translucent layer, a peptidoglycan layer and the plasma membrane, from outside to inside. A comparison of the structures of these two mycobacteria revealed that the M. leprae cell was smaller in size and had a thinner peptidoglycan layer than the M. tuberculosis cell. The cell widths measured on electron micrographs were 0.44 microm for M. tuberculosis and 0.38 microm for M. leprae. The peptidoglycan layer of M. leprae was 4-5 nm, while the corresponding layer of M. tuberculosis was 10-15 nm.
采用电子显微镜冷冻置换技术对麻风分枝杆菌泰国-53株的细胞壁和细胞质结构进行了研究，并与结核分枝杆菌H37Rv株进行了比较。两株菌的细胞壁结构均由外向内类似地由电子透明层、肽聚糖层和质膜组成多层结构。对这两种分枝杆菌结构的比较显示，麻风分枝杆菌细胞比结核分枝杆菌细胞体积更小，肽聚糖层更薄。在电子显微镜照片上测得的细胞宽度，结核分枝杆菌为0.44微米，麻风分枝杆菌为0.38微米。麻风分枝杆菌的肽聚糖层为4-5纳米，而结核分枝杆菌的相应层为10-15纳米。
