表达白细胞介素-4的质粒DNA可抑制DBA/1 J乳鼠中2型呼肠孤病毒引发的自身免疫性胰岛炎。
Interleukin-4-expressing plasmid DNA inhibits reovirus type-2-triggered autoimmune insulitis in DBA/1 J suckling mice.
作者信息
Hayashi T, Yasutomi Y, Hasegawa K, Sasaki Y, Onodera T
机构信息
Laboratory of Veterinary Pathology, Yamaguchi University, Yamaguchi, Japan.
出版信息
Int J Exp Pathol. 2003 Apr;84(2):101-6. doi: 10.1046/j.1365-2613.2003.00341.x.
Abstract
In this study we have examined the effect of systemic administration of T helper (Th) 2 cytokines on reovirus type-2 (Reo-2)-triggered Th1-mediated autoimmune insulitis with impaired glucose tolerance (IGT) in DBA/1J suckling mice. We have demonstrated clearly that the systemic administration of both interleukin (IL)-4-expressing plasmid DNA (pIL-4) and recombinant IL-4 (rIL-4) inhibited the development of insulitis with IGT in a dose dependent manner as compared to untreated groups in Reo-2-infected DBA/1J suckling mice. The inhibitory effects of IL-4 on the development of insulitis with IGT and the advantages of pIL-4 as compared to rIL-4 in this model are discussed.
摘要
在本研究中，我们检测了全身性给予辅助性T细胞（Th）2细胞因子对呼肠孤病毒2型（Reo-2）引发的、伴有葡萄糖耐量受损（IGT）的Th1介导的自身免疫性胰岛炎在DBA/1J乳鼠中的影响。我们已明确证明，与未处理组相比，在感染Reo-2的DBA/1J乳鼠中，全身性给予表达白细胞介素（IL）-4的质粒DNA（pIL-4）和重组IL-4（rIL-4）均以剂量依赖性方式抑制了伴有IGT的胰岛炎的发展。本文讨论了IL-4对伴有IGT的胰岛炎发展的抑制作用以及在此模型中pIL-4相对于rIL-4的优势。
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