Espina A I, Castellanos A V, Fereira J L
Laboratory of Forensic Odontology, Faculty of Odontology, Area of Pathology and Clinics, Institute of Investigations, University of Zulia, Venezuela.
Int Endod J. 2003 Jun;36(6):395-403. doi: 10.1046/j.1365-2591.2003.00659.x.
To describe the ultrastructural changes that occur in pulpal blood capillaries as a result of ageing.
Thirty samples of healthy dental pulps were obtained from functional human permanent teeth. Two age groups were examined: young (10-17 years) and old (>60 years). The teeth were extracted under local anaesthesia using mepivacaine without adrenaline (Scandonest 3%, Septodont, Saint-Maur des Fossés, France) and split longitudinally in a bench press. The pulps were gently removed, immersed in fixative solution, sectioned and processed by conventional transmission electron microscopic techniques. Micrographs were taken from the endothelium, and the whole capillary area of each vessel was examined.
In young pulps, the endothelial cell layer was characterized by the presence of numerous pinocytotic vesicles and microvesicles, RER cisterns, free ribosomes, a small Golgi complex, centrioles, microtubules, microfilaments and mitochondria. In the endothelial cell cytoplasm of older pulpal vessels, pinocytotic vesicles and microvesicles, as well as microfilaments, were more numerous. In addition, lipid-like vacuoles, monogranular glycogen granules and extensive Golgi complexes with dilated cisterns were also present. Weibel-Palade bodies were observed in both age groups without showing variations related with age.
The results obtained in capillaries of aged pulpal tissue suggest that the endothelium experiences morphological changes that could be associated with advancing age.
描述由于衰老而在牙髓毛细血管中发生的超微结构变化。
从功能正常的人类恒牙中获取30个健康牙髓样本。研究了两个年龄组：年轻组（10 - 17岁）和老年组（>60岁）。牙齿在局部麻醉下使用不含肾上腺素的甲哌卡因（斯康杜尼3%，Septodont公司，法国圣莫尔代福塞）拔除，并在台式压力机上纵向劈开。将牙髓轻轻取出，浸入固定液中，通过传统透射电子显微镜技术进行切片和处理。从内皮细胞拍摄显微照片，并检查每个血管的整个毛细血管区域。
在年轻牙髓中，内皮细胞层的特征是存在大量的吞饮小泡和微泡、粗面内质网池、游离核糖体、小型高尔基体复合体、中心粒、微管、微丝和线粒体。在老年牙髓血管的内皮细胞质中，吞饮小泡和微泡以及微丝数量更多。此外，还存在脂样空泡、单颗粒糖原颗粒和具有扩张池的广泛高尔基体复合体。在两个年龄组中均观察到了Weibel-Palade小体，且未显示出与年龄相关的变化。
在老年牙髓组织毛细血管中获得的结果表明，内皮细胞经历了可能与年龄增长相关的形态学变化。
