α6β1整合素是评估男性精子质量的潜在临床标志物。
Alpha 6 beta 1 Integrin is a potential clinical marker for evaluating sperm quality in men.
作者信息
Reddy Venkata Rami K, Rajeev Shrimali K, Gupta Vijayalakshmi
机构信息
Immunology Laboratory, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, India.
出版信息
Fertil Steril. 2003 Jun;79 Suppl 3:1590-6. doi: 10.1016/s0015-0282(03)00368-6.
OBJECTIVE
To determine the clinical significance of alpha6beta1 integrin in human spermatozoa.
DESIGN
Prospective case-controlled study.
SETTINGS
Hospital infertility clinic in India.
PATIENT(S): Sixty-two men undergoing evaluation for infertility and 20 healthy, proven-fertile controls were selected.
INTERVENTION(S): Role of alpha6beta1integrin in sperm quality evaluation was determined using semen analysis, cell ELISA, flow cytometry, and in vitro sperm-egg binding.
MAIN OUTCOME MEASURE(S): Determination of sperm-associated alpha6beta1 integrin may help in assessing the quality of the human spermatozoa.
RESULT(S): Expression of alpha6beta1 integrin was significantly low in the spermatozoa obtained from subfertile men. Only 35%-40% of spermatozoa from fertile men showed a positive reaction to alpha6beta1 antibody. The samples with high rate of acrosomal reaction showed a good expression of alpha6beta1 and maximum binding to oocyte in an in vitro binding assay.
CONCLUSION(S): alpha6beta1 Integrin may be used as a clinical marker to evaluate sperm quality.
目的
确定α6β1整合素在人类精子中的临床意义。
设计
前瞻性病例对照研究。
地点
印度的医院不孕不育诊所。
患者
选取62名接受不孕症评估的男性以及20名健康的、已证实有生育能力的对照者。
干预措施
通过精液分析、细胞酶联免疫吸附测定、流式细胞术和体外精卵结合来确定α6β1整合素在精子质量评估中的作用。
主要观察指标
测定与精子相关的α6β1整合素可能有助于评估人类精子的质量。
结果
从生育力低下男性获取的精子中，α6β1整合素的表达显著降低。来自有生育能力男性的精子中只有35%-40%对α6β1抗体呈阳性反应。在体外结合试验中，顶体反应率高的样本显示出α6β1的良好表达以及与卵母细胞的最大结合。
结论
α6β1整合素可用作评估精子质量的临床标志物。
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