Contera S Antoranz, Iwasaki H, Suzuki S
Interdisciplinary Nanoscience Centre, Institute of Physics and Astronomy, University of Aarhus, DK 8000, Aarhus C, Denmark.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):65-72. doi: 10.1016/S0304-3991(03)00030-5.
Trimeric Achromobacter cycloclastes Cu-containing nitrite reductase (CuNIR) proteins adsorbed on gold and graphite have been studied by ambient STM and in situ AFM. STM resolves them individually and in layers, distinguishing the sub-molecular individual units of the trimer. The Cu atoms are not visible to STM. STM shows that individual CuNIR denatures as it adsorbs on Au, although a deformed trimeric shape can be identified in some cases. CuNIR forms disordered layers on gold. On graphite, ordered self-assembled layers of CuNIR have been resolved by in situ AFM and ambient STM forming parallel rows whose separation distance corresponds to the size of one of the units of the trimer, 5nm. Ambient STM can achieve better resolution than in situ AFM in the images of the layers. We observe differences between domains showing the parallel row structure and unstructured parts of the CuNIR layer by in situ phase imaging AFM.
已通过环境扫描隧道显微镜（STM）和原位原子力显微镜（AFM）对吸附在金和石墨上的三聚体解环无色杆菌含铜亚硝酸盐还原酶（CuNIR）蛋白进行了研究。STM能够分辨出它们的单个分子以及分层结构，区分出三聚体的亚分子单个单元。STM无法看到铜原子。STM显示，单个CuNIR在吸附到金上时会变性，不过在某些情况下可以识别出变形的三聚体形状。CuNIR在金上形成无序层。在石墨上，原位AFM和环境STM已分辨出CuNIR的有序自组装层，形成平行排，其间距对应于三聚体其中一个单元的尺寸，即5纳米。在层的图像中，环境STM能比原位AFM实现更高的分辨率。通过原位相成像AFM，我们观察到显示平行排结构的区域与CuNIR层的非结构化部分之间存在差异。
