Osada Toshiya, Itoh Arimichi, Ikai Atsushi
Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, 226-8501, Yokohama, Japan.
Ultramicroscopy. 2003 Oct-Nov;97(1-4):353-7. doi: 10.1016/S0304-3991(03)00060-3.
The distribution of the receptor-associated protein (RAP) binding protein and the adhesion forces between RAP and its binding protein on living fibroblast cells were examined using an atomic force microscope (AFM). The distribution of RAP binding protein was obtained on 256 (16x16) locations in 2x2 micro m sections over the surface of living cells. The adhesion forces between RAP and the binding protein were measured with an AFM tip functionalized with RAP. In the presence of RAP in the scanning solution, the number of force curves with large adhesion force decreased. These results indicate that the adhesive forces observed here represent specific binding between RAP and the binding protein. This method will be a useful application of AFM to examine receptors on cell surfaces in high resolution.
使用原子力显微镜（AFM）检测了受体相关蛋白（RAP）结合蛋白的分布以及活成纤维细胞上RAP与其结合蛋白之间的粘附力。在活细胞表面2×2微米区域的256个（16×16）位置上获得了RAP结合蛋白的分布情况。用经RAP功能化的AFM探针测量了RAP与结合蛋白之间的粘附力。在扫描溶液中存在RAP的情况下，具有大粘附力的力曲线数量减少。这些结果表明，此处观察到的粘附力代表RAP与结合蛋白之间的特异性结合。该方法将是AFM在高分辨率检测细胞表面受体方面的一项有用应用。
