Bilsland James G, Harper Sarah J
Department of Biochemistry and Molecular Biology, Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK.
Neuroreport. 2003 May 23;14(7):995-9. doi: 10.1097/01.wnr.0000070828.57864.ad.
CEP-1347 inhibits the signalling pathway of c-jun-N-terminal kinase, and is neuroprotective in vivo and in vitro. Embryonic chick dorsal root ganglion neurones are dependent on NGF for survival and neurite outgrowth; NGF withdrawal results in apoptotic cell death. We examined the neuroprotective and neurite outgrowth promoting activity of CEP-1347 in dissociated DRG neurones and in primary DRG explants. CEP-1347 was as effective as NGF in promoting survival of dissociated DRG neurones, but caused only limited neurite outgrowth from DRG explants. When NGF was subsequently added to CEP-1347 treated explants, the outgrowth increased to a similar level to explants which had received NGF throughout. CEP-1347 may be a useful tool to maintain viable DRG explants to allow evaluation of neurite outgrowth promoting compounds and dissection of survival and neurite outgrowth signalling pathways.
CEP - 1347可抑制c - jun氨基末端激酶的信号通路，在体内和体外均具有神经保护作用。胚胎期鸡背根神经节神经元的存活和轴突生长依赖于神经生长因子（NGF）；去除NGF会导致细胞凋亡死亡。我们研究了CEP - 1347在解离的背根神经节（DRG）神经元和原代DRG外植体中的神经保护及促进轴突生长的活性。CEP - 1347在促进解离的DRG神经元存活方面与NGF效果相当，但仅能使DRG外植体产生有限的轴突生长。当随后向经CEP - 1347处理的外植体中添加NGF时，轴突生长增加至与全程接受NGF的外植体相似的水平。CEP - 1347可能是一种有用的工具，可用于维持存活的DRG外植体，以便评估促进轴突生长的化合物，并剖析存活和轴突生长信号通路。
