Skogh Charlotta, Campbell Kenneth
Wallenberg Neuroscience Center, Division of Neurobiology, Lund University, Sweden.
Neuroreport. 2003 May 23;14(7):1037-40. doi: 10.1097/01.wnr.0000073424.02536.22.
We have studied the differentiation of striatal projection neurons in co-culture with expanded glia from different regions of the embryonic mouse telencephalon. Our results show that when striatal progenitors are cultured on glia derived from the same region as they originate (i.e. the lateral ganglionic eminence), the neurons formed exhibit long processes. This is not the case when the cells are co-cultured with glia derived from the adjacent telencephalic region, the medial ganglionic eminence. Moreover, expression of the striatal projection neuron marker, dopamine- and cAMP-regulated phosphoprotein (DARPP-32) was significantly enhanced in neurons cultured on the homotopic glia. Thus, glial cultures derived from the lateral ganglionic eminence positively regulate the differentiation of striatal projection neurons in vitro.
我们研究了与来自胚胎小鼠端脑不同区域的扩增神经胶质细胞共培养时纹状体投射神经元的分化情况。我们的结果表明，当纹状体祖细胞在源自其起源相同区域（即外侧神经节隆起）的神经胶质细胞上培养时，形成的神经元具有长突起。而当细胞与源自相邻端脑区域内侧神经节隆起的神经胶质细胞共培养时，则并非如此。此外，在同型神经胶质细胞上培养的神经元中，纹状体投射神经元标志物多巴胺和cAMP调节磷蛋白（DARPP - 32）的表达显著增强。因此，源自外侧神经节隆起的神经胶质细胞培养物在体外对纹状体投射神经元的分化具有正向调节作用。
