Murakami T, Sasaki K, Takahashi M
Department of Pathology, Yamaguchi University School of Medicine.
Nihon Rinsho. 1992 Oct;50(10):2355-9.
Monoclonal antibody Ki-67 is a useful and easy means for evaluating cell proliferative activity. Furthermore, it appears to provide valuable prognostic informations in some neoplastic diseases. However, almost all studies on solid tumors were performed only by microscopic observation using immunohistochemistry, and not by flow cytometry. Therefore, the information deduced from the findings is limited. The method of DNA/Ki-67 double staining of solid tissue and cultured cells for flow cytometry is shown, and the significance and the usefulness of flow cytometric study of Ki-67 in clinical use are discussed.
单克隆抗体Ki-67是评估细胞增殖活性的一种有用且简便的方法。此外，它似乎能为某些肿瘤性疾病提供有价值的预后信息。然而，几乎所有关于实体瘤的研究都仅通过免疫组织化学显微镜观察进行，而非通过流式细胞术。因此，从这些发现中推断出的信息有限。本文展示了用于流式细胞术的实体组织和培养细胞DNA/Ki-67双重染色方法，并讨论了Ki-67流式细胞术研究在临床应用中的意义和实用性。
