Fontanini G, Pingitore R, Bigini D, Vignati S, Pepe S, Ruggiero A, Macchiarini P
Institute of Pathological Anatomy and Histology, University of Pisa, Italy.
Am J Pathol. 1992 Dec;141(6):1285-90.
Results generated by the immunohistochemical staining with PC10, a new monoclonal antibody recognizing PCNA (a nuclear protein associated with cell proliferation) in formalin-fixed and paraffin-embedded tissue were compared with those of Ki-67 labeling and DNA flow cytometry in 47 consecutive non-small cell lung cancer (NSCLC). PCNA reactivity was observed in all samples and confined to the nuclei of cancer cells. Its frequency ranged from 0 to 80% (37.7 +/- 23.6) and larger sized, early-staged and DNA aneuploid tumors expressed a significant higher number of PCNA-reactive cells. The PCNA and Ki-67 labeling rates were closely correlated (r = 0.383, P = 0.009). By flow cytometry, we observed a good correlation among PCNA labeling and S-phase fraction (r = 0.422, P = .0093) and G1 phase (r = 0.303, P = .051) of the cell cycle. Results indicate that PCNA labeling with PC10 is a simple method for assessing the proliferative activity in formalin-fixed, paraffin-embedded tissue of NSCLC and correlates well with Ki-67 labeling and S-phase fraction of the cell cycle.
在47例连续性非小细胞肺癌（NSCLC）中，将用PC10进行免疫组织化学染色产生的结果与Ki-67标记和DNA流式细胞术的结果进行了比较。PC10是一种识别PCNA（一种与细胞增殖相关的核蛋白）的新型单克隆抗体，用于福尔马林固定、石蜡包埋组织。在所有样本中均观察到PCNA反应性，且局限于癌细胞的细胞核。其频率范围为0至80%（37.7±23.6），较大尺寸、早期阶段和DNA非整倍体肿瘤表达的PCNA反应性细胞数量显著更高。PCNA和Ki-67标记率密切相关（r = 0.383，P = 0.009）。通过流式细胞术，我们观察到PCNA标记与细胞周期的S期分数（r = 0.422，P = 0.0093）和G1期（r = 0.303，P = 0.051）之间存在良好的相关性。结果表明，用PC10进行PCNA标记是评估NSCLC福尔马林固定、石蜡包埋组织中增殖活性的一种简单方法，并且与Ki-67标记和细胞周期的S期分数密切相关。
