Lee Seung-Hee, Han Jin-Hee, Choi Jung-Hwan, Huh Eun-Young, Kwon Yunhee Kim, Kaang Bong-Kiun
National Research Laboratory of Neurobiology, Institute of Molecular Biology and Genetics, School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.
Mol Cells. 2003 Apr 30;15(2):233-9.
Brain-derived neurotrophic factor (BDNF) plays a key role in the differentiation and neuritogenesis of developing neurons, and in the synaptic plasticity of mature neurons, in the mammalian nervous system. BDNF binds to the receptor tyrosine kinase TrkB and transmits neurotrophic signals by activating neuron-specific tyrosine phosphorylation pathways. However, the neurotrophic function of BDNF in Aplysia neurons is poorly understood. We examined the specific effect of BDNF on neurite outgrowth and synaptic plasticity in cultured Aplysia neurons and a multipotent rat hippocampal stem cell line (HiB5). Our study indicates that mammalian BDNF has no significant effect on the neuritogenesis, neurotransmitter release, excitability, and synaptic plasticity of cultured Aplysia neurons in our experimental conditions. In contrast, BDNF in combination with platelet-derived growth factor (PDGF) increases the length of the neurites and the number of spine-like structures in cells of HiB5.
脑源性神经营养因子（BDNF）在哺乳动物神经系统中，对发育中神经元的分化和神经突发生，以及成熟神经元的突触可塑性起着关键作用。BDNF与受体酪氨酸激酶TrkB结合，并通过激活神经元特异性酪氨酸磷酸化途径来传递神经营养信号。然而，人们对BDNF在海兔神经元中的神经营养功能了解甚少。我们研究了BDNF对培养的海兔神经元和多能大鼠海马干细胞系（HiB5）中神经突生长和突触可塑性的具体影响。我们的研究表明，在我们的实验条件下，哺乳动物BDNF对培养的海兔神经元的神经突发生、神经递质释放、兴奋性和突触可塑性没有显著影响。相反，BDNF与血小板衍生生长因子（PDGF）联合使用可增加HiB5细胞中神经突的长度和棘状结构的数量。
