Rünzi M, Müller M K, Schmid P, von Schönfeld J, Goebell H
Department of Medicine, University of Essen, Germany.
Pancreas. 1992;7(6):619-23. doi: 10.1097/00006676-199211000-00001.
The influence of the neuropeptide galanin, present in intrapancreatic nerve endings, on the endocrine pancreas is well known. The most potent effect of galanin is inhibition of insulin release. Little is known of its effect on the exocrine pancreas. Whether galanin plays a role in the regulation of exocrine pancreatic secretion and whether this effect is mediated directly on acinar cells or indirectly via the influence on insulin secretion is not clear. In the present study, we investigated these questions using the model of the isolated and arterially perfused rat pancreas with intact exocrine and endocrine secretion. In the presence of 15.8 mM glucose in a modified Krebs-Ringer buffer and during half-maximal stimulation of enzyme secretion with 100 pmol/ml cholecystokinin octapeptide (CCK-8), a dose-response study of 0.001-100 pmol/ml porcine galanin was performed. At concentrations of 0.001 and 0.01 pmol/ml, porcine galanin significantly stimulated insulin release (p < 0.05 and < 0.01, respectively) and also significantly enhanced CCK-8-stimulated amylase secretion (p < 0.05). Doses of 0.1 and 1 pmol/ml galanin resulted in a nonsignificant inhibition of insulin release, while 10 and 100 pmol/ml strongly inhibited the endocrine response (p < 0.001). However, concentration levels of 1-100 pmol/ml galanin did not affect CCK-8-stimulated amylase secretion. Rat galanin, tested at 0.01 and 10 pmol/ml, showed no significant difference from the effects of porcine galanin at the equipotent concentrations. It is concluded that the effect of galanin on exocrine pancreas, like the effect on endocrine functions, tends to be a direct one and that it could exert a modulatory influence on the level of neuronal transmission.
存在于胰腺内神经末梢的神经肽甘丙肽对内分泌胰腺的影响已为人熟知。甘丙肽最显著的作用是抑制胰岛素释放。其对外分泌胰腺的作用却鲜为人知。甘丙肽是否在调节外分泌胰腺分泌中发挥作用，以及这种作用是直接作用于腺泡细胞还是通过对胰岛素分泌的影响间接发挥作用尚不清楚。在本研究中，我们使用具有完整外分泌和内分泌分泌功能的离体动脉灌注大鼠胰腺模型来研究这些问题。在改良的 Krebs-Ringer 缓冲液中存在 15.8 mM 葡萄糖的情况下，并用 100 pmol/ml 八肽胆囊收缩素（CCK-8）进行酶分泌的半最大刺激时，进行了 0.001 - 100 pmol/ml 猪甘丙肽的剂量反应研究。在 0.001 和 0.01 pmol/ml 的浓度下，猪甘丙肽显著刺激胰岛素释放（分别为 p < 0.05 和 < 0.01），并且还显著增强了 CCK-8 刺激的淀粉酶分泌（p < 0.05）。0.1 和 1 pmol/ml 的甘丙肽剂量导致胰岛素释放的抑制不显著，而 10 和 100 pmol/ml 则强烈抑制内分泌反应（p < 0.001）。然而，1 - 100 pmol/ml 的甘丙肽浓度水平并未影响 CCK-8 刺激的淀粉酶分泌。以 0.01 和 10 pmol/ml 测试的大鼠甘丙肽，在等效浓度下与猪甘丙肽的作用无显著差异。得出的结论是，甘丙肽对外分泌胰腺的作用，如同对内分泌功能的作用一样，倾向于是直接的，并且它可能在神经元传递水平上发挥调节作用。
