孕期表达的一种乳腺衍生生长抑制因子（MDGI）相关多肽的纯化。

Purification of a mammary-derived growth inhibitor (MDGI) related polypeptide expressed during pregnancy.

作者信息

Brandt R, Grosse R

机构信息

Max-Delbrück-Center for Molecular Medicine, Berlin-Buch, Germany.

出版信息

Biochem Biophys Res Commun. 1992 Nov 30;189(1):406-13. doi: 10.1016/0006-291x(92)91573-9.

DOI:10.1016/0006-291x(92)91573-9

PMID:1280424

Abstract

The present study was undertaken to screen immunochemically for MDGI-related proteins in the mammary gland. A new form, MDGI 2, not present in lactation could be detected in the bovine gland during pregnancy. It was further distinguished from MDGI by its lower molecular weight, its association with a complex binding to WGA, and by lacking immunoreactivity to an anti-MDGI antibody directed against the C-terminus of MDGI. MDGI 2 was purified by chromatography over DEAE-Sepharose, Bio-Gel P-30 in 1% acetic acid, Sephacryl S-200 in 6 M urea and Mono Q. Final purification included HPLC on TSK G-3000 SW and electroelution from SDS-gels. Cell-free translation of poly (A+)mRNA from glands of pregnant animal yielded one form identical with MDGI. We assume that posttranslational processing of MDGI is involved in its activities.

摘要

本研究旨在通过免疫化学方法筛选乳腺中与MDGI相关的蛋白质。在怀孕期的牛乳腺中可检测到一种哺乳期不存在的新形式，即MDGI 2。它与MDGI的进一步区别在于分子量较低，与WGA有复杂结合，且对针对MDGI C末端的抗MDGI抗体缺乏免疫反应性。通过在DEAE-琼脂糖、1%乙酸中的Bio-Gel P-30、6 M尿素中的Sephacryl S-200和Mono Q上进行层析，对MDGI 2进行纯化。最终纯化包括在TSK G-3000 SW上进行HPLC以及从SDS凝胶上进行电洗脱。对怀孕动物乳腺的多聚（A+）mRNA进行无细胞翻译，产生了一种与MDGI相同的形式。我们认为MDGI的翻译后加工涉及其活性。