Baliga B S, Isoyama K, Longmore G, Yang Y M, Shah A K, Mankad V N
Comprehensive Sickle Cell Center, University of Southern Alabama Medical Center, Mobile 36617.
Blood Cells. 1992;18(2):187-93; discussion 194-5.
A simple method of hemoglobin analysis in a cell culture system is described. Hemoglobins synthesized in cell cultures are labeled with radioactive amino acids. The cell extract containing radiolabeled hemoglobin is mixed with A, F, S, C, hemoglobin markers and separated by cellulose acetate electrophoresis. Individual bands of hemoglobin are cut from the gel and analyzed for radioactivity. This method is especially useful for determination of newly synthesized minute amount of hemoglobin in cell extracts that are difficult to visualize by staining procedure.
本文描述了一种在细胞培养系统中进行血红蛋白分析的简单方法。在细胞培养中合成的血红蛋白用放射性氨基酸进行标记。将含有放射性标记血红蛋白的细胞提取物与A、F、S、C血红蛋白标志物混合，并通过醋酸纤维素电泳进行分离。从凝胶上切下各个血红蛋白条带并分析其放射性。该方法对于测定细胞提取物中新合成的微量血红蛋白特别有用，这些微量血红蛋白难以通过染色程序进行可视化。
