Wlodarczyk B, Biernacki B, Minta M, Zmudzki J
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland.
ScientificWorldJournal. 2001 Jun 5;1:227-34. doi: 10.1100/tsw.2001.48.
Postimplantation whole embryo culture (WEC) assay for rats and mice has been well established and introduced to many laboratories. Recently WEC technique for rabbits has been developed; however, information on culture of other species is very limited. Knowing the usefulness of hamsters in classical embryotoxicology, we reasoned that hamster WEC could be an alternative model for the most frequently used rat and mouse WEC. Previously we have optimized culture conditions for postimplantation hamster embryos. The aim of this study was to test the susceptibility of hamster embryos cultured in vitro to embryotoxic compounds and to compare our results with those reported by others on rat or mouse embryo culture. For that purpose we choose three known embryotoxic compounds--valproic acid, cadmium chloride, and diethylstilbestrol--and tested them using a postimplantation hamster whole embryo culture assay. Hamster embryos were cultured from 7.5 days gestation for 24 h in a medium consisting of 35% hamster serum and 65% synthetic culture medium (Iscove's or McCoy 5A). At the end of the culture period, the embryos were examined morphologically, measured with the aid of a computer image analysis system, and total protein content was assessed. All three compounds exhibited dose-related embryotoxic and teratogenic effects in hamster embryos. The malformations observed were similar to those reported on rat and mouse embryos. Comparison of the results with data reported by other authors indicates that hamster embryos cultured in vitro might be more susceptible to embryotoxic stimuli than rat and mouse embryos.
大鼠和小鼠的植入后全胚胎培养（WEC）试验已经得到充分确立并被引入到许多实验室。最近，兔的WEC技术也已开发出来；然而，关于其他物种培养的信息非常有限。鉴于仓鼠在经典胚胎毒理学中的用途，我们推断仓鼠WEC可能是最常用的大鼠和小鼠WEC的替代模型。此前我们已经优化了植入后仓鼠胚胎的培养条件。本研究的目的是测试体外培养的仓鼠胚胎对胚胎毒性化合物的敏感性，并将我们的结果与其他人在大鼠或小鼠胚胎培养方面报道的结果进行比较。为此，我们选择了三种已知的胚胎毒性化合物——丙戊酸、氯化镉和己烯雌酚——并使用植入后仓鼠全胚胎培养试验对它们进行测试。仓鼠胚胎在妊娠7.5天时开始培养，在由35%仓鼠血清和65%合成培养基（伊斯科夫培养基或麦科伊5A培养基）组成的培养基中培养24小时。在培养期结束时，对胚胎进行形态学检查，借助计算机图像分析系统进行测量，并评估总蛋白含量。所有三种化合物在仓鼠胚胎中均表现出剂量相关的胚胎毒性和致畸作用。观察到的畸形与在大鼠和小鼠胚胎上报道的相似。将结果与其他作者报告的数据进行比较表明，体外培养的仓鼠胚胎可能比大鼠和小鼠胚胎对胚胎毒性刺激更敏感。
