哺乳动物培养细胞的匀浆化
Homogenization of mammalian cultured cells.
作者信息
Graham John M
机构信息
School of Biomolecular Sciences, Liverpool John Moores University.
出版信息
ScientificWorldJournal. 2002 Jun 13;2:1630-3. doi: 10.1100/tsw.2002.848.
Abstract
Satisfactory homogenization of cultured cells is a necessary prerequisite to any fractionation schedule. Protocols are given for homogenization in iso-osmotic (A) and hypo-osmotic (B) media that should be broadly applicable to any cell type and to any subsequent fractionation procedure. Alternative procedures are also summarized in the Notes section, but detailed operation of some of the automated devices is beyond the scope of this short Protocol Article.
摘要
培养细胞的充分匀浆是任何分级分离方案的必要前提。本文给出了在等渗（A）和低渗（B）介质中进行匀浆的方案，这些方案应广泛适用于任何细胞类型和任何后续的分级分离程序。替代程序也在注释部分进行了总结，但一些自动化设备的详细操作超出了本简短方案文章的范围。
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