Neethling W M L, Hodge A J, Glancy R
Fremantle Heart Institute & University of Western Australia, Fremantle Hospital, Fremantle, Western Australia.
J Biomed Mater Res B Appl Biomater. 2003 Jul 15;66(1):356-63. doi: 10.1002/jbm.b.10015.
Stentless aortic heart valve substitutes, manufactured from biological tissues, are fixed with glutaraldehyde to cross-link collagen, reduce antigenicity, and sterilize the tissue. Despite improved cross linking, reduced antigenicity, and various anticalcification measures, the aortic wall tissue present in these prostheses tends to calcify. The aim of this study was to assess the morphology, collagen cross-link stability, and calcification potential of glutaraldehyde-preserved kangaroo aortic wall tissue as opposed to porcine aortic wall tissue. Porcine and kangaroo aortic wall tissues were fixed in 0.625% buffered glutaraldehyde. Histology and cross-link stability were examined. Calcification potential was determined in the subcutaneous rat model. Kangaroo aortic wall tissue was significantly (p < 0.01) less calcified than porcine aortic wall tissue (26.67 +/- 6.53 versus 41.959 +/- 2.75 microg/mg tissue) at 8 weeks. In conclusion, the histological differences between kangaroo and porcine aortic wall tissue correlate well with the reduced calcification potential of kangaroo aortic wall tissue. The reduced calcification potential could result in improved long-term durability of stentless kangaroo heart valves as bioprostheses.
由生物组织制成的无支架主动脉心脏瓣膜替代品，用戊二醛固定以交联胶原蛋白、降低抗原性并对组织进行消毒。尽管交联有所改善、抗原性降低且采取了各种抗钙化措施，但这些假体中存在的主动脉壁组织仍有钙化倾向。本研究的目的是评估与猪主动脉壁组织相比，戊二醛保存的袋鼠主动脉壁组织的形态、胶原蛋白交联稳定性和钙化潜力。将猪和袋鼠的主动脉壁组织固定在0.625%的缓冲戊二醛中。检查组织学和交联稳定性。在大鼠皮下模型中测定钙化潜力。在8周时，袋鼠主动脉壁组织的钙化程度明显低于猪主动脉壁组织（26.67±6.53对41.959±2.75微克/毫克组织，p<0.01）。总之，袋鼠和猪主动脉壁组织之间的组织学差异与袋鼠主动脉壁组织较低的钙化潜力密切相关。钙化潜力的降低可能会提高无支架袋鼠心脏瓣膜作为生物假体的长期耐久性。
