鸡胚胎发育过程中cVg1 mRNA的表达
Expression of cVg1 mRNA during chicken embryonic development.
作者信息
Somi Semir, Houweling Arjan C, Buffing Anita A M, Moorman Antoon F M, Van Den Hoff Maurice J B
机构信息
Experimental and Molecular Cardiology Group, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
出版信息
Anat Rec A Discov Mol Cell Evol Biol. 2003 Jul;273(1):603-8. doi: 10.1002/ar.a.10070.
Using degenerated PCR-primers to identify known and novel BMPs that are expressed in the developing chicken heart, we identified not only BMP2, -4, and -7 mRNA, but also the TGFbeta superfamily member cVg1. The expression pattern of cVg1 mRNA was determined during chicken development from HH4 to HH44. In early developmental stages, cVg1 mRNA is expressed in the primitive streak, paraxial mesoderm, developing somites, and developing neural tube. Subsequently, cVg1 mRNA is expressed in the developing central and peripheral nervous system, retina, auditory vesicle, notochord, lung alveoli, and olfactory mucosa. In the heart, cVg1 is initially expressed through the linear heart tube, but becomes restricted to the forming chamber myocardium, in an expression domain similar to that of atrial natriuretic factor (ANF) mRNA.
利用简并PCR引物来鉴定在发育中的鸡心脏中表达的已知和新型骨形态发生蛋白（BMP），我们不仅鉴定出了BMP2、-4和-7的mRNA，还鉴定出了转化生长因子β（TGFβ）超家族成员cVg1。在鸡从HH4到HH44的发育过程中确定了cVg1 mRNA的表达模式。在发育早期阶段，cVg1 mRNA在原条、近轴中胚层、发育中的体节和发育中的神经管中表达。随后，cVg1 mRNA在发育中的中枢和外周神经系统、视网膜、听泡、脊索、肺泡和嗅觉粘膜中表达。在心脏中，cVg1最初通过线性心管表达，但在类似于心钠素（ANF）mRNA的表达域中，其表达局限于正在形成的心室心肌。
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