Somi Semir, Buffing Anita A M, Moorman Antoon F M, Van Den Hoff Maurice J B
Experimental and Molecular Cardiology Group, Cardiovascular Research Institute Amsterdam, Academic Medical Center, Amsterdam, The Netherlands.
Anat Rec A Discov Mol Cell Evol Biol. 2004 Jul;279(1):579-82. doi: 10.1002/ar.a.20052.
In this communication we describe the expression pattern of BMP10 mRNA during cardiac development in chickens. BMP10 is considered an important factor in the regulation of cardiac growth and trabeculation in the murine embryo. We identified chicken Ests, which are similar to mouse and human BMP10 in the UMIST database. The cDNA clone that contained most sequences was obtained, verified by sequence analysis, and used to determine the spatiotemporal pattern of gene expression. BMP10 mRNA is initially expressed at HH10 in the myocardium of the arterial pole of the heart tube, anterior to the interventricular groove. Between HH14 and HH22, BMP10 mRNA becomes broadly expressed in the outflow tract, the distal part of the inflow tract, and the trabeculated part of the developing ventricles and atria. From HH31 onward, BMP10 mRNA expression decreases in the ventricular myocardium by first disappearing from the compact myocardium and then from the tips of the trabecules. At HH44, BMP10 mRNA is expressed only in the trabeculated myocardium of the atria and the endocardium of the ventricles. The observed expression pattern of BMP10 mRNA suggests that it may play a role in regulating the formation of the ventricular wall and trabecules.
在本交流中，我们描述了鸡心脏发育过程中BMP10 mRNA的表达模式。BMP10被认为是调节小鼠胚胎心脏生长和小梁形成的重要因素。我们在UMIST数据库中鉴定出与小鼠和人类BMP10相似的鸡ESTs。获得了包含大部分序列的cDNA克隆，通过序列分析进行了验证，并用于确定基因表达的时空模式。BMP10 mRNA最初在心脏管动脉极的心肌中于HH10时表达，位于室间沟前方。在HH14至HH22之间，BMP10 mRNA在流出道、流入道远端以及发育中的心室和心房的小梁化部分广泛表达。从HH31开始，BMP10 mRNA在心室心肌中的表达减少，首先从致密心肌中消失，然后从小梁尖端消失。在HH44时，BMP10 mRNA仅在心房的小梁化心肌和心室的心内膜中表达。观察到的BMP10 mRNA表达模式表明它可能在调节心室壁和小梁的形成中起作用。
