小鼠Ifi200基因簇：基因组序列、分析及与人类HIN-200基因簇的比较。

The mouse Ifi200 gene cluster: genomic sequence, analysis, and comparison with the human HIN-200 gene cluster.

作者信息

Deschamps Stéphane, Meyer Jeffrey, Chatterjee Gouri, Wang Hong, Lengyel Peter, Roe Bruce A

机构信息

Department of Chemistry and Biochemistry, University of Oklahoma, 620 Parrington Oval, Norman, OK 73019, USA.

出版信息

Genomics. 2003 Jul;82(1):34-46. doi: 10.1016/s0888-7543(03)00092-2.

DOI:10.1016/s0888-7543(03)00092-2

PMID:12809674

Abstract

The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203', Ifi204, and Ifi204') in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204' mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.

摘要

干扰素可激活的Ifi200基因簇位于小鼠1号染色体的1q21 - q23区域。我们在此报告对129/SvJ小鼠中两个基因组区域的分析，这两个区域编码至少10个密切相关的200家族基因（Ifi201、Ifi202a、Ifi202b、Ifi202c、Ifi203a、Ifi203b、Ifi203c、Ifi203'、Ifi204和Ifi204'）。通过基于BAC的测序方法，获得了这些高度相似的Ifi200基因的确切结构和组织。在Ifi202a和b之间以及Ifi203a和b之间观察到高度保守性（99%的同一性）。Ifi203a和b的内含子4中存在一个额外的转录区域，提示存在可变剪接的可能性，并且Ifi204' mRNA的一个剪接变体与一个相关但未定位的D3 mRNA表现出91%的序列同一性。小鼠和人类基因簇的比较分析表明，非编码序列中不存在显著的序列保守性，这表明200家族在人类和小鼠物种形成之前就已出现，随后在基因复制后发生了分化。