Jee Kyoung Soo, Kim Yong Soo, Park Ki Dong, Kim Young Ha
Department of Molecular Science and Technology, Ajou University, San 5, Wonchon-Dong, Paldal-Gu, Suwon 442-749, South Korea.
Biomaterials. 2003 Sep;24(20):3409-16. doi: 10.1016/s0142-9612(03)00204-7.
A novel chemical modification of biological tissues was developed by the direct coupling of bioactive molecule, L-arginine to bovine pericardium (BP). The modification involves pretreatment of BP using GA and followed by grafting arginine to BP by the reaction of residual aldehyde and amine group of L-arginine. BP was modified by direct coupling of bioactive molecules and the effect of L-arginine coupling on calcification and biocompatibility was evaluated in vitro and in vivo. Modified BPs were characterized by measuring shrinkage temperature, mechanical properties, digestion resistance to collagenase enzyme, in vitro plasma protein adsorption and platelet adhesion, and in vivo calcification. Thermal and mechanical properties showed that the durability of arginine treated tissue increased as compared with fresh tissue and GA treated tissue. Resistance to collagenase digestion revealed that modified tissues have greater resistance to enzyme digestion than did fresh tissue and GA treated tissue. Lower protein adsorption and platelet adhesion were observed on modified tissue than non-modified tissue. In vivo calcification study demonstrated much less calcium deposition on arginine treated BP than GA treated one. Obtained results attest to the usefulness of L-arginine treated BP for cardiovascular bioprostheses.
通过将生物活性分子L-精氨酸直接偶联到牛心包（BP）上，开发了一种新型的生物组织化学修饰方法。该修饰包括先用戊二醛（GA）预处理BP，然后通过L-精氨酸的残留醛基和胺基反应将精氨酸接枝到BP上。通过生物活性分子的直接偶联对BP进行修饰，并在体外和体内评估L-精氨酸偶联对钙化和生物相容性的影响。通过测量收缩温度、力学性能、对胶原酶的消化抗性、体外血浆蛋白吸附和血小板粘附以及体内钙化来表征修饰后的BP。热性能和力学性能表明，与新鲜组织和GA处理的组织相比，精氨酸处理的组织的耐久性有所提高。对胶原酶消化的抗性表明，修饰后的组织比新鲜组织和GA处理的组织对酶消化具有更大的抗性。与未修饰的组织相比，在修饰后的组织上观察到较低的蛋白质吸附和血小板粘附。体内钙化研究表明，精氨酸处理的BP上的钙沉积比GA处理的BP少得多。所得结果证明L-精氨酸处理的BP用于心血管生物假体是有用的。
