Evaluation of a new pretreatment reagent for use with the EMIT 2000 cyclosporine assay.
作者信息
Butch Anthony W, Fukuchi Alan M
机构信息
Department of Pathology & Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1713, USA.
出版信息
Clin Biochem. 2003 Jun;36(4):313-6. doi: 10.1016/s0009-9120(03)00011-0.
Abstract
OBJECTIVES
Evaluate the performance of the new pretreatment (NPT) reagent for use with the EMIT cyclosporine A (CsA) assay.
DESIGN AND METHODS
Samples from transplant patients receiving CsA were tested using a COBAS MIRA S.
RESULTS
A downward shift in target values for commercial controls was observed using the NPT reagent. There was excellent correlation for patient results when comparing the NPT reagent with methanol pretreatment.
CONCLUSION
The NPT reagent is an acceptable substitute for methanol in the EMIT(R) extraction procedure for CsA.
摘要
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