[重组人转化生长因子β3对成纤维细胞作用的观察]

[An observation of the effects of recombinant human transforming growth factor beta3 on fibroblast].

作者信息

Lu Luo, Chen Yu-Lin, Zhang Qing-Guo

机构信息

Department of Burns, Changhai Hospital, The Second Military Medical University, Shanghai 200433. P.R. China.

出版信息

Zhonghua Shao Shang Za Zhi. 2003 Apr;19(2):97-9.

PMID:12812635

Abstract

OBJECTIVE

To investigate the role of recombinant human transforming growth factor beta3 (rhTGFbeta3) on fibroblast and its possible mechanism.

METHODS

Normal skin fibroblast (NSFb) and hypertrophic scar fibroblast (HSFb) were cultured in vitro, and were processed by different concentrations of rhTGFbeta3. NSFb and HSFb in DMEM solution without rhTGFbeta3 were employed as control. The changes in the protein and mRNA expression of type I and III collagen in NSFb and HSFb were observed.

RESULTS

(1) The expression of type I and III procollagen in NSFb was evidently different from that of HSFb (2) The synthesis of type I and III procollagen in all test groups was increased obviously after rhTGFbeta3 process (P < 0.001) while the ratio of type I to III procollagen was decreased when compared with that in control group. (3) The effects of rhTGFbeta3 on the biological behavior exhibited an obvious dose- effects relationship. The contents of type I to III procollagen in HSFb were higher than those in NSFb when the dose of rhTGFbeta3 was same.

CONCLUSION

rhTGFbeta3 could effectively promote the synthesis of type I and III procollagen, especially type III procollagen in fibroblasts. This might be beneficial to the accelerate of wound healing and to inhibit or prevent scar formation.

摘要

目的

探讨重组人转化生长因子β3（rhTGFβ3）对成纤维细胞的作用及其可能机制。

方法

体外培养正常皮肤成纤维细胞（NSFb）和增生性瘢痕成纤维细胞（HSFb），用不同浓度的rhTGFβ3处理，以不含rhTGFβ3的DMEM溶液中的NSFb和HSFb作为对照。观察NSFb和HSFb中Ⅰ型和Ⅲ型胶原蛋白的蛋白质和mRNA表达变化。

结果

（1）NSFb中Ⅰ型和Ⅲ型前胶原蛋白的表达与HSFb明显不同；（2）rhTGFβ3处理后，所有试验组中Ⅰ型和Ⅲ型前胶原蛋白的合成均明显增加（P < 0.001），而与对照组相比，Ⅰ型与Ⅲ型前胶原蛋白的比例降低；（3）rhTGFβ3对生物学行为的影响呈现明显的剂量-效应关系。当rhTGFβ3剂量相同时，HSFb中Ⅰ型至Ⅲ型前胶原蛋白的含量高于NSFb。