Zhang Cheng-de, Tian Ying, Song Lan, Zhang Cai-ping
Department of Burns and Plastic Surgery, The First People' s Hospital of Changde City, China.
Zhonghua Zheng Xing Wai Ke Za Zhi. 2005 Nov;21(6):440-4.
To investigate the influence of adrenaline on the expression of TGFbeta1, bFGF and procollagen for human normal and hypertrophic scar dermal fibroblasts cultured in vitro.
Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-beta1 and I procollagen in fibroblasts were determined by RT-PCR. Levels of bFGF and TGF-beta1 in the supernatants of fibroblasts cultured in vitro were determined by enzyme-linked immunosorbent assay (ELISA).
In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines (P < 0.01). It also caused statistically significant decrease in the level of TGF-beta1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0.10 and 0.20 micromol/L adrenaline resulted in a statistically significant (P < 0.01) decrease in the expression of I procollagen mRNA. However, only 0.20 micromol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts.
We conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-beta1 and I procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.
研究肾上腺素对体外培养的人正常及增生性瘢痕皮肤成纤维细胞中转化生长因子β1（TGFβ1）、碱性成纤维细胞生长因子（bFGF）和前胶原表达的影响。
将人正常及增生性瘢痕皮肤成纤维细胞在含肾上腺素的无血清体外模型中培养24小时。通过逆转录聚合酶链反应（RT-PCR）测定成纤维细胞中bFGF、TGF-β1和I型前胶原的人mRNA水平。采用酶联免疫吸附测定（ELISA）法测定体外培养的成纤维细胞上清液中bFGF和TGF-β1的水平。
在我们的研究中，肾上腺素使正常及增生性瘢痕成纤维细胞系的bFGF峰值水平出现统计学显著升高（P < 0.01）。它还使正常及增生性瘢痕成纤维细胞系的TGF-β1水平出现统计学显著降低。用0.05、0.10和0.20微摩尔/升肾上腺素处理正常成纤维细胞，导致I型前胶原mRNA表达出现统计学显著降低（P < 0.01）。然而，只有0.20微摩尔/升肾上腺素能降低增生性瘢痕成纤维细胞中I型前胶原的mRNA表达。
从这些结果我们得出结论，肾上腺素可增加体外培养的人正常皮肤及增生性瘢痕成纤维细胞中bFGF的产生，并降低TGF-β1和I型前胶原的产生。
