Koval'chuk O
Ukr Biokhim Zh (1978). 1992 Sep-Oct;64(5):23-30.
Ribosomes can have two states at 0 degree C: competent and noncompetent in translocation. In both states poly(U)-programmed ribosomes bind phenylalanyl-tRNA to A and P sites and form peptide bond. Elongation factor G promotes fast translocation in competent ribosomes and makes them noncompetent ones. Initial correlation between competent and noncompetent ribosomes is 2:1. Addition of deacylated tRNA does not influence phenomenon described as well as thermal reactivation of the ribosomes before beginning of the experiments. The possibility of deacylated tRNA translocation is shown. The translocation does not occurred provided that at least one of the ribosome sites is filled with shortened tRNA analog (tRNA with truncated CCA-end or tRNA anticodon arm).
核糖体在0摄氏度时可处于两种状态：转位时具有活性和无活性。在这两种状态下，聚尿苷酸（poly(U)）编程的核糖体将苯丙氨酰-tRNA结合到A位点和P位点并形成肽键。延伸因子G促进有活性核糖体的快速转位并使其变为无活性核糖体。有活性和无活性核糖体之间的初始比例为2:1。去酰化tRNA的添加以及实验开始前核糖体的热再激活均不影响上述现象。已证明去酰化tRNA具有转位的可能性。如果核糖体的至少一个位点被缩短的tRNA类似物（具有截短CCA末端的tRNA或tRNA反密码子臂）占据，则不会发生转位。
