Robbins J, McFadzean I, Brown D A
Department of Pharmacology, University College London, U.K.
Agents Actions Suppl. 1992;38 ( Pt 2):98-107.
Under whole-cell recording, bradykinin (BK) produced an initial outward membrane current followed by an inward current in voltage-clamped NG108-15 cells. The initial outward current was associated with a rise in intracellular Ca2+ and was accompanied by the opening of Ca(2+)-dependent K(+)-channels recorded with a cell-attached patch electrode. This current was inhibited by intracellular Mg2+. The inward current was associated with inhibition of the voltage-dependent K(+)-current IK(M). These effects accord with those previously observed in microelectrode-impaled cells, with the difference that BK produced much more pronounced and long-lasting desensitization in the patch-clamped cells.
在全细胞记录条件下，缓激肽（BK）在电压钳制的NG108 - 15细胞中产生了一个初始外向膜电流，随后是内向电流。初始外向电流与细胞内Ca2+升高相关，并伴随着用细胞贴附式膜片电极记录到的Ca(2 +)依赖性K(+)通道的开放。该电流被细胞内Mg2+抑制。内向电流与电压依赖性K(+)电流IK(M)的抑制相关。这些效应与先前在微电极刺入细胞中观察到的一致，不同之处在于BK在膜片钳制细胞中产生了更明显和持久的脱敏作用。
