Kramer T R, Grossniklaus H E, Vigneswaran N, Waring G O, Kozarsky A
Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia.
Invest Ophthalmol Vis Sci. 1992 Dec;33(13):3581-5.
The immunocytologic characteristics of two formalin-fixed, paraffin-embedded corneas from patients with the iridocorneal endothelial (ICE) syndrome and unaffected control corneas were studied. Binding of polyclonal antisera to Factor VIII, S-100 protein, involucrin, neuron specific enolase (NSE), and the lectins peanut agglutinin and Ulex europaeus agglutinin-1 was performed using the standard peroxidase-anti-peroxidase method. We detected reactive patterns of monoclonal antibodies to cytokeratins (34BE12 is a 56-58 kD mouse IgG reactive to stratified epithelia; Pkk1 is a 44-54 kD mouse IgG reactive to simple epithelia; and KL1 is a 55-57 kD mouse IgG reactive to epidermis and simple epithelia) using the standard avidin-biotin complex method. Staining properties were similar for the polyclonal antisera, lectins, NSE, and chromogranin in corneas with ICE syndrome and in the controls. However, the cytokeratins 34BE12, Pkk1, and KL1 were detected in the endothelium of the corneas with the ICE syndrome but not in the controls. These findings suggest that various cytokeratins are expressed in the corneal endothelium in the ICE syndrome that are not expressed in unaffected corneal endothelium.
对来自虹膜角膜内皮（ICE）综合征患者的两片福尔马林固定、石蜡包埋角膜以及未受影响的对照角膜的免疫细胞特征进行了研究。使用标准的过氧化物酶 - 抗过氧化物酶方法进行多克隆抗血清与因子VIII、S - 100蛋白、外皮蛋白、神经元特异性烯醇化酶（NSE）以及凝集素花生凝集素和欧洲荆豆凝集素 - 1的结合。我们使用标准的抗生物素蛋白 - 生物素复合物方法检测了针对细胞角蛋白的单克隆抗体的反应模式（34BE12是一种对复层上皮有反应的56 - 58 kD小鼠IgG；Pkk1是一种对单层上皮有反应的4-54 kD小鼠IgG；KL1是一种对表皮和单层上皮有反应的55 - 57 kD小鼠IgG）。ICE综合征角膜和对照角膜中多克隆抗血清、凝集素、NSE和嗜铬粒蛋白的染色特性相似。然而，在ICE综合征角膜的内皮中检测到了细胞角蛋白34BE12、Pkk1和KL1，而在对照角膜中未检测到。这些发现表明，ICE综合征中角膜内皮表达了多种在未受影响的角膜内皮中不表达的细胞角蛋白。
