Murtha Jill M, Keller Evan T
Unit for Laboratory Animal Medicine and Department of Pathology, University of Michigan, Box 0940 CCGCB, Room 5304, 1500 East Medical Center Drive, Ann Arbor, MI 48109-0940, USA.
Exp Gerontol. 2003 Jun;38(6):683-91. doi: 10.1016/s0531-5565(03)00067-6.
Heat shock proteins (Hsps) are involved in many physiological and pathological processes and are diminished with age in a variety of species. As zebrafish embryos have proven to be excellent models for studying Hsp response during development, we sought to characterize the response in mature zebrafish to demonstrate the utility of the zebrafish model in studying late-life diseases and the biology of aging. Accordingly, mature zebrafish were exposed to a 37 degrees C heat stress and mRNA was isolated from various tissues and subjected to analysis by RT-PCR. We found that Hsp70 was upregulated in brain, liver, and muscle, while Hsp47 was upregulated in brain, but not liver or muscle. Hsp90alpha, Hsp90beta, and heat shock factor 1a (Hsf1a) were expressed in all three tissues, but were not upregulated in response to heat stress. A comparison of Hsp expression in young versus mature zebrafish revealed decreased basal levels of Hsp70 and increased levels of Hsf1a in mature fish. These results indicate that the heat shock response is detectable in mature zebrafish and that there are age differences in their heat shock response, suggesting that mature zebrafish may be a useful model for studying Hsp response during the aging process.
热休克蛋白（Hsps）参与许多生理和病理过程，并且在多种物种中会随着年龄增长而减少。由于斑马鱼胚胎已被证明是研究发育过程中热休克蛋白反应的优秀模型，我们试图对成熟斑马鱼的反应进行表征，以证明斑马鱼模型在研究晚年疾病和衰老生物学方面的实用性。因此，将成熟斑马鱼暴露于37摄氏度的热应激下，从各种组织中分离出mRNA，并通过逆转录聚合酶链反应（RT-PCR）进行分析。我们发现，热休克蛋白70（Hsp70）在脑、肝脏和肌肉中上调，而热休克蛋白47（Hsp47）仅在脑中上调，在肝脏或肌肉中未上调。热休克蛋白90α（Hsp90α）、热休克蛋白90β（Hsp90β）和热休克因子1a（Hsf1a）在所有三种组织中均有表达，但对热应激无上调反应。对年轻斑马鱼和成熟斑马鱼的热休克蛋白表达进行比较发现，成熟斑马鱼中热休克蛋白70的基础水平降低，热休克因子1a的水平升高。这些结果表明，在成熟斑马鱼中可检测到热休克反应，并且它们的热休克反应存在年龄差异，这表明成熟斑马鱼可能是研究衰老过程中热休克蛋白反应的有用模型。
