Mariani S M, Armandola E A, Ferrone S
Department of Microbiology and Immunology, New York Medical College, Valhalla 10595.
Transplantation. 1992 Dec;54(6):1078-84. doi: 10.1097/00007890-199212000-00024.
A total of 630 hybridomas were generated from a BALB/c mouse immunized with the anti-id mAb F5-444, which binds an idiotope within the antigen-combining site of mAb AC1.59. The latter recognizes a determinant shared by HLA-DR1, DRw8, DR9 antigens and subtypes of HLA-DR4 and DR6 that is poorly expressed by HLA-DRw16 and DRw17 antigens. Eight anti-anti-id mAb were shown with serological and immunochemical assays to react with HLA-DR antigens. Detailed analysis of these anti-HLA-DR anti-anti-id mAb showed that they differ from the original anti-HLA-DR mAb AC1.59 and among themselves in either isotype, fine specificity, extent of reactivity with the nominal antigen, differential reactivity with soluble or cell-bound antigen, and/or idiotype profile. These observations emphasize the need to characterize a panel of antigen-binding anti-anti-id mAb in order to evaluate the degree of similarity existing between antigen-binding antibodies induced by an anti-id mAb and the original antigen-binding mAb.
用抗独特型单克隆抗体F5 - 444免疫BALB/c小鼠，共产生了630个杂交瘤，F5 - 444可结合单克隆抗体AC1.59抗原结合位点内的一个独特型表位。后者识别HLA - DR1、DRw8、DR9抗原以及HLA - DR4和DR6亚型所共有的一个决定簇，而该决定簇在HLA - DRw16和DRw17抗原上表达较弱。通过血清学和免疫化学分析表明，有8种抗抗独特型单克隆抗体与HLA - DR抗原发生反应。对这些抗HLA - DR抗抗独特型单克隆抗体的详细分析表明，它们在同种型、精细特异性、与标称抗原的反应程度、与可溶性或细胞结合抗原的差异反应性和/或独特型谱方面，与原始抗HLA - DR单克隆抗体AC1.59不同，彼此之间也存在差异。这些观察结果强调，需要对一组抗原结合抗抗独特型单克隆抗体进行表征，以评估由抗独特型单克隆抗体诱导的抗原结合抗体与原始抗原结合单克隆抗体之间存在的相似程度。
