Simakova A V, Pankova T F, Issi I V
Parazitologiia. 2003 Mar-Apr;37(2):145-53.
Microsporidia parasitizing the adipose body of mosquito larvae of Anopheles beklemishevi and Aedes punctor has been studied. Two new genera of microsporidia are described based on lightmicroscopic and ultrastructural characteristics of spores and sporogony stages. The spore wall of Crepidula beklemishevi gen. n. et sp. n. is formed by two-membrane exospore, thick exospore, bilayer endospore and thin plasmolemma. Spores with single nucleus, polar filament anisofilar, with 6-7 coils (2+ 4-5), polaroplast consisting of three parts: macrochelicoidal, microhelicoidal and lamellar. Fixed spores 4.2 +/- 0.22 x 2 +/- 0.01 microns. The sporogony of Dimeiospora palustris gen. et. n. results in spore formation of two different types. Spores of the first type are oviform, with thick wall, single-nuclear, 6.1 x 4.9 microns. Spore wall with three layers, about 370 nm. Exospore electron-dense, subexospore moderately electrondense. Exospore and subexospore irregularly pleated on the almost spore surface and slightly thinner on anterior end only. Endospore electron-translucent. Polar filament anisofilar, with 9 coils (3 + 6). Polaroplas consists of three parts: lamellar, fine bubbled, and coarse bubbled. Spores of the second type broad-ovate, with apical pole narrower, distal pole concave, 4.6 x 3.7 microns. Spore wall with three layer, 355 nm. Exospore on the apical end irregularly pleated, consists of thin electrondense exospore, subexospore of variable electron density, endospore electron-translucent. Polar filament anisofilar, with 13 coils (3 + 10). Polaroplast has two parts: lamellar and vesicular.
对寄生于贝氏按蚊和尖音库蚊幼虫脂肪体的微孢子虫进行了研究。基于孢子和孢子生殖阶段的光学显微镜和超微结构特征，描述了两个微孢子虫新属。贝氏克氏微孢子虫属（新属）及新种的孢子壁由双膜外孢子、厚外孢子、双层内膜和薄质膜组成。单核孢子，极丝异丝，有6 - 7圈（2 + 4 - 5），极质体由三部分组成：大螯状、小螯状和片状。固定孢子大小为4.2±0.22×2±0.01微米。沼泽双形微孢子虫属（新属）的孢子生殖产生两种不同类型的孢子。第一种类型的孢子呈卵形，壁厚，单核，大小为6.1×4.9微米。孢子壁有三层，约370纳米。外孢子电子致密，亚外孢子中等电子致密。外孢子和亚外孢子在几乎整个孢子表面不规则褶皱，仅前端稍薄。内膜电子透明。极丝异丝，有9圈（3 + 6）。极质体由三部分组成：片状、细泡状和粗泡状。第二种类型的孢子宽卵形，顶端极窄，远端极凹陷，大小为4.6×3.7微米。孢子壁有三层，355纳米。顶端的外孢子不规则褶皱，由薄的电子致密外孢子、电子密度可变的亚外孢子和电子透明的内膜组成。极丝异丝，有13圈（3 + 10）。极质体有两部分：片状和泡状。
