Torres Marysabel, Márquez Mercedes, Sutil Rosalía, Carrizales Maira, Leal María, Reigosa Aldo
Department of Pharmacology, School of Medicine, University of Carabobo, Valencia, Venezuela.
Invest Clin. 2003 Jun;44(2):119-27.
The purpose of this study was to determine whether the administration of Vitamin E (200 mg/day) for 4 weeks could decrease hypercholesterolemia and inhibit atherosclerosis in male hypercholesterolemic New Zealand White rabbits. Plasmatic Total Cholesterol (TC) and LDL-Cholesterol (LDLc) were determined by enzymatic methods and Vitamin E was determined in plasma by HPLC at weeks 0, 4 and 8. A histopathological study was carried out at week 8 (w 8), using a hematoxylin-eosin method. Forty rabbits were divided randomly into 5 groups and fed different diets. These diets included a Normal diet, a 1% Cholesterol rich diet, a Normal diet + Vitamin E and 1% Cholesterol rich diet + Vitamin E. The findings did not show a reduction of Total Cholesterol and LDLc in the groups of rabbits that received Vitamin E (III and IV) at weeks 4 and 8. At the end of the experiment, all the animals were deeply anesthetized with hydrochloride ketamina (60 mg/kg body wt) in order to carry out a histopathological study. Regarding the atherosclerotic lesions, Vitamin E did not induce inhibition of the atherosclerotic plaque development or any modification in the lesion type induced by the hypercholesterolemic diet. In conclusion, the results suggest that additional studies need to be carried out with higher doses and/or treatments for a longer period in order to clarify in detail whether Vitamin E really has a hypocholesterolemic effect and inhibits atherosclerosis.
本研究的目的是确定给予雄性高胆固醇血症新西兰白兔维生素E（200毫克/天）4周是否能降低高胆固醇血症并抑制动脉粥样硬化。在第0、4和8周，通过酶法测定血浆总胆固醇（TC）和低密度脂蛋白胆固醇（LDLc），并通过高效液相色谱法测定血浆中的维生素E。在第8周（w 8）采用苏木精-伊红染色法进行组织病理学研究。40只兔子随机分为5组，给予不同饮食。这些饮食包括正常饮食、富含1%胆固醇的饮食、正常饮食+维生素E以及富含1%胆固醇的饮食+维生素E。研究结果显示，在第4周和第8周接受维生素E的兔子组（III组和IV组）中，总胆固醇和LDLc并未降低。实验结束时，为了进行组织病理学研究，所有动物均用氯胺酮盐酸盐（60毫克/千克体重）深度麻醉。关于动脉粥样硬化病变，维生素E并未抑制动脉粥样硬化斑块的发展，也未对高胆固醇饮食诱导的病变类型产生任何改变。总之，结果表明需要进行更高剂量和/或更长时间治疗的进一步研究，以详细阐明维生素E是否真的具有降胆固醇作用并抑制动脉粥样硬化。
