Li Ya-jie, Liu Li, Zhang Feng-chun
Department of Rheumatology and Immunology, Union Hospital of Peking Union Medical College, Chinese Academy of Medical Science, Beijing 100730, China.
Zhonghua Nei Ke Za Zhi. 2003 Mar;42(3):165-8.
To distinguish the difference among the pathogenesis of 60 000 SSA antigen mono-antigen peptides (MAPs), and to discuss the nosogenesis of anti-60 000 SSA antibodies in correlative rheumatic diseases.
MAPs were artificially synthesized according to the amino acid sequence of 20 positive epitopes and 1 control segment of 60 000 SSA antigen. ELISA against recombinant 60 000 SSA antigen MAPs were done to detect anti-MAPs antibodies in 59 sera with anti-SSA antibodies, and analyzing the relations of anti-MAPs antibodies and organism injuries.
Anti-60 000 SSA antibodies are multiple clone autoantibodies. Different patients have different immune response to MAPs. Anti-MAP(1 approximately 21) antibodies have no relation to skin lesion; anti-MAP(2,14,15,21) antibodies have positive relation to salivary gland lesion; anti-MAP(7,16) antibodies have negative relation to kidney lesion; anti-MAP(6) antibodies have negative relation to heart lesion.
The appearance of some anti-MAPs antibodies implies the lesions of some organs, while the appearance of some other anti-MAPs antibodies have the protection to some organs. We concluded that it is maybe the different anti-MAPs antibodies that result in different clinical manifestations.
鉴别60 000 SSA抗原单抗原肽（MAPs）致病机制的差异，探讨相关风湿性疾病中抗60 000 SSA抗体的发病机制。
根据60 000 SSA抗原的20个阳性表位氨基酸序列及1个对照片段人工合成MAPs。采用针对重组60 000 SSA抗原MAPs的酶联免疫吸附测定（ELISA）检测59份含抗SSA抗体血清中的抗MAPs抗体，并分析抗MAPs抗体与机体损伤的关系。
抗60 000 SSA抗体为多克隆自身抗体。不同患者对MAPs有不同免疫反应。抗MAP(1～21)抗体与皮肤损害无关；抗MAP(2、14、15、21)抗体与唾液腺损害呈正相关；抗MAP(7、16)抗体与肾脏损害呈负相关；抗MAP(6)抗体与心脏损害呈负相关。
部分抗MAPs抗体的出现预示某些器官损害，而部分抗MAPs抗体的出现对某些器官有保护作用。我们推断可能是不同的抗MAPs抗体导致了不同的临床表现。
