Fuchshuber P R, Lotzová E
Department of General Surgery, University of Texas, M.D. Anderson Cancer Center, Houston 77030.
Lymphokine Cytokine Res. 1992 Oct;11(5):271-6.
The aim of this study was to characterize the oncolytic efficacy of human natural killer (NK) cell subsets generated from highly NK-enriched population in long-term IL-2 cultures. NK cells cultured for 3 weeks with interleukin-2 (IL-2) were separated into several subsets using two color fluorescence-activated cell sorting and CD2, CD8, CD16, and CD56 monoclonal antibodies. These individual NK cell subsets were then tested for cytotoxicity against various tumor target cell lines, including K-562, Daudi, and Ovcar-3. The CD16+/CD56+ NK cell subset was superior in its cytotoxic activity against all targets in comparison to the CD16-/CD56+ subset. Within CD16 population, CD16+CD2+ NK cells were most potent; however CD16+/CD2- subset was also cytotoxic, indicating that CD2 molecule is important, but not necessary for NK cell cytotoxic function. CD16+/CD8+ and CD16+/CD8- subsets showed variance in cytolytic efficacy, depending on the tumor target tested. Highest cytotoxicity against K-562 was observed in the CD16+/CD8+ population, while the CD16+/CD8- subset manifested highest cytotoxic activity against Daudi. No significant differences within these NK cell subsets were observed against Ovcar-3 targets. These data indicate that NK cell subsets are not equally oncolytic, and that the oncolytic effect may be tumor dependent.
本研究的目的是对在长期白细胞介素-2（IL-2）培养中从高度富集自然杀伤（NK）细胞的群体中产生的人NK细胞亚群的溶瘤效力进行表征。用白细胞介素-2（IL-2）培养3周的NK细胞使用双色荧光激活细胞分选和CD2、CD8、CD16和CD56单克隆抗体被分离成几个亚群。然后测试这些单个NK细胞亚群对包括K-562、Daudi和Ovcar-3在内的各种肿瘤靶细胞系的细胞毒性。与CD16-/CD56+亚群相比，CD16+/CD56+NK细胞亚群对所有靶标的细胞毒性活性更强。在CD16群体中，CD16+CD2+NK细胞最具活性；然而，CD16+/CD2-亚群也具有细胞毒性，这表明CD2分子对于NK细胞的细胞毒性功能很重要，但不是必需的。CD16+/CD8+和CD16+/CD8-亚群的溶细胞效力存在差异，这取决于所测试的肿瘤靶标。在CD16+/CD8+群体中观察到对K-562的最高细胞毒性，而CD16+/CD8-亚群对Daudi表现出最高的细胞毒性活性。在针对Ovcar-3靶标的这些NK细胞亚群中未观察到显著差异。这些数据表明，NK细胞亚群的溶瘤作用并不相同，并且溶瘤效果可能取决于肿瘤类型。
