豚鼠黑质致密部神经元体外瞬时外向整流对离子通道阻断剂的敏感性。

Sensitivity of transient outward rectification to ion channel blocking agents in guinea-pig substantia nigra pars compacta neurones in vitro.

作者信息

Harris N C

机构信息

University Department of Pharmacology, Oxford, UK.

出版信息

Brain Res. 1992 Nov 20;596(1-2):325-9. doi: 10.1016/0006-8993(92)91566-w.

DOI:10.1016/0006-8993(92)91566-w

PMID:1281747

Abstract

The sensitivity of a transient outward rectifier in guinea-pig substantia nigra pars compacta (SNC) neurones in vitro was tested to a variety of ion channel blocking agents. This rectification was not blocked by TEA (10 mM), 4-aminopyridine (4-AP) (5 mM), apamin (0.2-1 microM) or Cd2+ ions (200 microM). Cs+ ions (5 mM) blocked the inward 'anomalous' rectifier but had no effect on the transient outward rectification. However, replacing Ca2+ ions in the bath perfusate by Ba2+ blocked the transient outward but not the inward rectifier. It is suggested that an atypical barium-sensitive conductance is responsible for the transient outward rectification of these neurones.

摘要

在体外对豚鼠黑质致密部（SNC）神经元中瞬时外向整流器的敏感性进行了测试，使用了多种离子通道阻断剂。这种整流不受10 mM四乙铵（TEA）、5 mM 4-氨基吡啶（4-AP）、0.2 - 1 microM蜂毒明肽或200 microM Cd2+离子的阻断。5 mM Cs+离子阻断内向“异常”整流，但对瞬时外向整流无影响。然而，用Ba2+替代浴灌流液中的Ca2+离子可阻断瞬时外向整流而非内向整流。提示一种非典型的钡敏感电导负责这些神经元的瞬时外向整流。