Taniguchi Masatoshi, Nagai Koji, Arao Nakako, Kawasaki Tomihisa, Saito Tetsu, Moritani Yumiko, Takasaki Jun, Hayashi Kazumi, Fujita Shigeo, Suzuki Ken-ichi, Tsukamoto Shin-ichi
Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21, Miyukigaoka, Tsukuba-shi, Ibaraki 305-8585, Japan.
J Antibiot (Tokyo). 2003 Apr;56(4):358-63. doi: 10.7164/antibiotics.56.358.
A novel platelet aggregation inhibitor, YM-254890, was isolated from the culture broth of strain QS3666. This strain was isolated from a soil sample collected at Okutama, Tokyo, Japan, and was identified as Chromobacterium sp. by morphological and physiological criteria. YM-254890 was purified from the culture supernatant by solvent extraction, ODS and silica gel flash chromatography, followed by preparative HPLC. YM-254890 inhibited ADP-induced platelet aggregation in human platelet-rich plasma with an IC50 value below 0.6 microM by blocking the P2Y1 receptor-signal transduction pathway.
一种新型血小板聚集抑制剂YM-254890，是从菌株QS3666的培养液中分离得到的。该菌株是从日本东京奥多摩采集的土壤样本中分离出来的，通过形态学和生理学标准鉴定为嗜铬杆菌属。YM-254890通过溶剂萃取、ODS和硅胶快速柱色谱，随后进行制备型高效液相色谱，从培养上清液中纯化得到。YM-254890通过阻断P2Y1受体信号转导途径，抑制富含人血小板血浆中ADP诱导的血小板聚集，IC50值低于0.6微摩尔。
