白细胞介素-2抑制已鉴定的海兔神经元中γ-氨基丁酸诱导的氯离子电流。

Interleukin-2 inhibits the GABA-induced Cl- current in identified Aplysia neurons.

作者信息

Sawada M, Hara N, Ichinose M

机构信息

Department of Physiology, Shimane Medical University, Izumo, Japan.

出版信息

J Neurosci Res. 1992 Nov;33(3):461-5. doi: 10.1002/jnr.490330312.

DOI:10.1002/jnr.490330312

PMID:1281891

Abstract

The effects of extracellularly applied recombinant human interleukin-2 (rhIL-2) on the gamma-aminobutyric acid (GABA)-induced Cl- current recorded from identified neurons (R9 and R12) of Aplysia kurodai were investigated with conventional voltage-clamp and pressure ejection techniques. Bath-applied rhIL-2 (10-40 U/ml) reduced the GABA-induced current in the neurons without affecting resting membrane conductance and the holding current. The suppressing effect of rhIL-2 on the current was completely reversible. Heat-inactivated rhIL-2 was without effect. These results suggest that the immunomodulator IL-2 can modulate the GABA-induced response in the nervous system.

摘要

采用传统电压钳和压力喷射技术，研究了细胞外施加重组人白细胞介素-2（rhIL-2）对黑海天牛（Aplysia kurodai）已鉴定神经元（R9和R12）中γ-氨基丁酸（GABA）诱导的氯离子电流的影响。浴槽中施加rhIL-2（10-40 U/ml）可降低神经元中GABA诱导的电流，而不影响静息膜电导和钳制电流。rhIL-2对电流的抑制作用是完全可逆的。热灭活的rhIL-2则无作用。这些结果表明，免疫调节剂IL-2可调节神经系统中GABA诱导的反应。