Aoki Kyoko, Suzuki Takeshi, Murakami Noriaki
Department of Botany, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
J Plant Res. 2003 Aug;116(4):337-44. doi: 10.1007/s10265-003-0103-9. Epub 2003 Jun 19.
For the purpose of phylogeographic study of lucidophyllous (evergreen broad-leaved) forests in Japan, we surveyed intraspecific chloroplast DNA (cpDNA) variation in 41 component species of such forests. Intraspecific cpDNA variations were detected in 14 species. In 15 species and one species group, 16 non-coding cpDNA regions were examined to find intraspecific sequence variation. The extent of variation in these regions was compared. The largest amount of intraspecific variation was detected in the rps16 region. A relatively large amount of intraspecific variation was detected in the petD-rpoA, rpl16, and trnL-F regions. It is suggested that these regions of cpDNA would be useful for detecting intraspecific variation in plant species, and could provide valuable information for various research purposes.
为了对日本的常绿阔叶林进行系统发育地理学研究，我们调查了这类森林41个组成物种的种内叶绿体DNA（cpDNA）变异情况。在14个物种中检测到了种内cpDNA变异。在15个物种和1个物种组中，对16个非编码cpDNA区域进行了检测以寻找种内序列变异。比较了这些区域的变异程度。在rps16区域检测到的种内变异量最大。在petD-rpoA、rpl16和trnL-F区域检测到了相对较大的种内变异量。这表明cpDNA的这些区域对于检测植物物种的种内变异很有用，并且可以为各种研究目的提供有价值的信息。
