Miura Masaru, Ichikawa Yasushi, Tanaka Kuniya, Kamiyama Masako, Hamaguchi Youhei, Ishikawa Takashi, Yamaguchi Shigeki, Togo Shinji, Ike Hideyuki, Ooki Shigeo, Shimada Hiroshi
Department of Surgery-II, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, Japan, 236-0004.
Anticancer Res. 2003 Mar-Apr;23(2B):1271-6.
Reverse transcriptase-polymerase chain reaction (RT-PCR) has been utilized to detect living micrometastases of cancer cells in the lymph node, ascites or circulation system. However, the method was so sensitive that false-positives happened frequently. Therefore we have developed a quantification of CEA mRNA using real-time PCR to detect living cancer cells in the circulating blood and examined its usefulness as a predictive marker for liver metastases of colon cancer. In cell spiking experiments, it was possible to detect CEA mRNA in 10(1) cancer cells diluted in 10(7) normal lymphocytes. In the blood samples of cancer patients, the CEA mRNA level was significantly higher in Dukes' D patients than in the other clinical stages of colorectal cancer. This indicates that quantification of CEA mRNA is useful for the evaluation of colorectal cancer progress and that the post-operative increase of CEA mRNA can be a predictive marker for micrometastasis.
逆转录聚合酶链反应（RT-PCR）已被用于检测淋巴结、腹水或循环系统中癌细胞的存活微转移。然而，该方法过于敏感，假阳性频繁发生。因此，我们开发了一种使用实时PCR定量检测循环血液中存活癌细胞的CEA mRNA的方法，并检验了其作为结肠癌肝转移预测标志物的实用性。在细胞掺入实验中，能够在10⁷个正常淋巴细胞中稀释的10¹个癌细胞中检测到CEA mRNA。在癌症患者的血液样本中，Dukes' D期患者的CEA mRNA水平明显高于其他临床分期的结直肠癌患者。这表明CEA mRNA定量对于评估结直肠癌进展是有用的，并且术后CEA mRNA的升高可以作为微转移的预测标志物。
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