Katsumata Kenji, Sumi Tetsuo, Mori Yasuharu, Hisada Masayuki, Tsuchida Akihiko, Aoki Tatsuya
The Third Department of Surgery, Tokyo Medical University, 6-7-1 Nishi-shinjuku, Shinjuku-ku, Tokyo, 160-0022, Japan.
Int J Clin Oncol. 2006 Oct;11(5):385-9. doi: 10.1007/s10147-006-0590-5.
As a mode of colorectal cancer recurrence, liver metastasis plays an important role. One of the factors reported to predict liver metastasis is the detection of trace amounts of tumor cells in the blood. For this purpose, cancer cell-induced cytokeratins (CKs) are generally identified, using the reverse transcriptase-polymerase chain reaction (RT-PCR). In the present study, we aimed to detect trace amounts of tumor cells, based on CK20, in the circulating venous blood, and we examined pathological factors, liver metastasis, and prognosis.
The subjects were 57 colorectal cancer patients who had undergone operation. We examined the cancer-induced marker (CK20) in circulating venous blood by RT-PCR and investigated the relationships between this marker, pathological factors, and prognosis.
Detection ratio of CK20 mRNA was 42.1%, and CK20 was significantly correlated with the pathological factor of lymph node metastasis (P = 0.037). The 5-year survival rate for CK20-positive patients was 62.5% and that for the CK20-negative patients was 87.5%; there was a significant difference (P = 0.048) between the two groups. Recurrence was recognized in six patients; two were positive for CK20 and four were negative for CK20.
These findings indicate that CK20 is strongly related to lymph node metastasis and prognosis, suggesting its usefulness for the diagnosis of colorectal cancer recurrence. However, CK20 did not predict liver metastasis.
作为结直肠癌复发的一种方式，肝转移起着重要作用。据报道，预测肝转移的因素之一是检测血液中微量的肿瘤细胞。为此，通常使用逆转录聚合酶链反应（RT-PCR）来鉴定癌细胞诱导的细胞角蛋白（CKs）。在本研究中，我们旨在基于CK20检测循环静脉血中的微量肿瘤细胞，并研究病理因素、肝转移和预后情况。
研究对象为57例接受手术的结直肠癌患者。我们通过RT-PCR检测循环静脉血中的癌症诱导标志物（CK20），并研究该标志物、病理因素与预后之间的关系。
CK20 mRNA的检测率为42.1%，CK20与淋巴结转移的病理因素显著相关（P = 0.037）。CK20阳性患者的5年生存率为62.5%，CK20阴性患者的5年生存率为87.5%；两组之间存在显著差异（P = 0.048）。6例患者出现复发；2例CK20阳性，4例CK20阴性。
这些发现表明CK20与淋巴结转移和预后密切相关，提示其对结直肠癌复发诊断的有用性。然而，CK20不能预测肝转移。
