Li Wei-min, Wang Su-min, Pei Xiu-ying, Liu Zhong-quan, Zhong Qiu, Qian Ming, Zhao Bing, Duanmu Hong-jin
Beijing Tuberculosis and Chest Tumor Institution, Beijing 101149, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 May;24(5):381-4.
To explore the epidemic distribution of Mycobacterium tuberculosis isolates from Beijing, Guangdong and Ningxia, and to determine M. tuberculosis strains of the "Beijing Family".
Two hundred and six IS6110 DNA fingerprinting patterns of M. tuberculosis strains from three provinces (city) were transferred to digital data, compared with the world M. tuberculosis DNA fingerprinting database, and then clustered by Gel compare 4.1 software. The clustering values in different patients with tuberculosis were compared by chi(2) test. Risk factors for recent transmission were calculated using odd ratios.
No M. tuberculosis strains were found the same as those of DNA fingerprint database. 56.8% (117/206) fingerprinting patterns of M. tuberculosis shared by least two-thirds of the IS6110 fragments and their Spoligotyping fingerprinting patterns were consistent with those of M.tuberculosis strains of the "Beijing Family". There were significant differences between female and male, different age groups (< 42 years old) and older (>or= 42 years old) (P < 0.05). Odd ratio was 5.06 in the group younger than 42 years old (95% CI: 1.00 - 34.34) and was 4.43 (95% CI: 0.94 - 28.76) in males.
M. tuberculosis strains of "Beijing Family" were popular in Beijing, Guangdong and Ningxia. Men and younger age group (< 42) were shown to be infected by identical strains more often than women and older aged which might play an important role in the recent transmission of tuberculosis in these areas. IS6110 DNA fingerprinting of M. tuberculosis could be used to trace the source of tuberculosis infection.
探讨北京、广东和宁夏结核分枝杆菌分离株的流行分布情况，并确定“北京家族”结核分枝杆菌菌株。
将来自三省（市）的206株结核分枝杆菌菌株的IS6110 DNA指纹图谱转化为数字数据，与全球结核分枝杆菌DNA指纹图谱数据库进行比较，然后用Gel compare 4.1软件进行聚类分析。采用卡方检验比较不同结核病患者的聚类值。使用比值比计算近期传播的危险因素。
未发现与DNA指纹数据库相同的结核分枝杆菌菌株。56.8%（117/206）的结核分枝杆菌指纹图谱至少有三分之二的IS6110片段相同，且其间隔寡核苷酸分型指纹图谱与“北京家族”结核分枝杆菌菌株一致。女性与男性、不同年龄组（<42岁）和年龄较大组（≥42岁）之间存在显著差异（P<0.05）。42岁以下组的比值比为5.06（95%可信区间：1.00 - 34.34），男性为4.43（95%可信区间：0.94 - 28.76）。
“北京家族”结核分枝杆菌菌株在北京、广东和宁夏流行。男性和年龄较小组（<42岁）比女性和年龄较大者更易感染相同菌株，这可能在这些地区结核病的近期传播中起重要作用。结核分枝杆菌的IS6110 DNA指纹图谱可用于追踪结核病感染源。
