He Min, Zeng Er-liang, Zheng Yan-yan, Tang Zhuo, Lu Xiang-chan, Sun Bi-hui, Xu Ding-kong, Zhang Zhi-yong, Yang Li
Medical Scientific Research Center, Guangxi Medical University, Nanning 530021, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2003 May;24(5):385-8.
To evaluate the gene-chip detecting rifaman-resistance Mycobacterium tuberculosis applied in TB diagnosis and drug-resistant detection.
Mycobacterium tuberculosis and rifaman-resistant strains among 35 rifaman-resistance isolated strains and 102 sputa specimens from TB patients, 27 sputa specimens from other patients were examined the gene-chips. Results obtained were compared with sputum examination, bacteriological culture and standard drug susceptibility test of Mycobacterium tuberculosis.
Thirty-five rifaman-resistance strains were detected by gene-chips and 33 were identified as rifaman-resistance strains and the concordance with the traditional drug susceptibility test of Mycobacterium tuberculosis was 94.29%. Twenty-seven sputa specimens from other patients were examined Mycobacterium tuberculosis by the gene-chips, 2 were positive, the detection specialty was 92.59%. Using three methods detecting Mycobacterium tuberculosis among 102 sputa specimens the positive rate respectively was, sputum examination 35.29% (36/102), bacteriological culture 28.43% (29/102), gene-chip 77.45% (79/102). Among 102 sputa specimens only 29 examined Mycobacterium tuberculosis by the traditional drug susceptibility test and 8 were rifaman-resistant strains. While using gene-chip, there were 20 among 102 sputa specimens identified as rifaman-resistance strains. Among total 55 rifaman-resistance strains detected by the gene-chips, the most frequent mutations were those associated with codon 531 (23 of 55; 41.8%), 526 (15 of 55; 27.27%) and 516 (9 of 55; 16.36%).
Results showed that this was a rapid, simple and highly specific method when using gene-chip to detect Mycobacterium tuberculosis and rifaman-resistant strains.
评估基因芯片检测利福平耐药结核分枝杆菌在结核病诊断及耐药检测中的应用。
对35株利福平耐药分离株、102份结核病患者痰液标本及27份其他患者痰液标本中的结核分枝杆菌及利福平耐药菌株进行基因芯片检测。将所得结果与结核分枝杆菌痰液检查、细菌培养及标准药敏试验结果进行比较。
基因芯片检测出35株利福平耐药菌株，其中33株被鉴定为利福平耐药菌株，与传统结核分枝杆菌药敏试验的一致性为94.29%。基因芯片检测27份其他患者痰液标本中的结核分枝杆菌，2份呈阳性，检测特异性为92.59%。采用三种方法检测102份痰液标本中的结核分枝杆菌，阳性率分别为：痰液检查35.29%（36/102），细菌培养28.43%（29/102），基因芯片77.45%（79/102）。102份痰液标本中仅29份进行了传统药敏试验检测结核分枝杆菌，其中8株为利福平耐药菌株。而采用基因芯片检测时，102份痰液标本中有20株被鉴定为利福平耐药菌株。在基因芯片检测出的55株利福平耐药菌株中，最常见的突变与密码子531（55株中的23株；41.8%）、526（55株中的15株；27.27%）和516（55株中的9株；16.36%）相关。
结果表明，基因芯片检测结核分枝杆菌及利福平耐药菌株是一种快速、简便且特异性高的方法。
