Fasske E, Fetting R, Rühland D, Schubert T, Themann H
Z Krebsforsch Klin Onkol Cancer Res Clin Oncol. 1975 Nov 25;84(3):251-69. doi: 10.1007/BF00312247.
Cells of a virogenic, immature myelogenic leukemia were injected in i.p. in one week old mice, strain NMRI, each animal receiving 10(5), 10(6) or 13(7) cells respectively in 0.2 ml Hanks' BSS. Mice were sacrificed at different times between 10 hrs and 21 days p.i. and liver specimens were prepared for electronmicroscopic studies. 10 hrs p.i. the leukemic cells are found in the sinusoids of the liver and after 30 hrs in the periportal fields. The leukemia cells migrate into the Disse spaces from the sinusoids. The leukemic cells penetrate with cytoplasmic digitations between connected endothelial cells. There is no lytic disintegration of the endothelial cells. The leukemic cells multiply by mitotic division of the Disse spaces, thereby compressing the liver cells. The cell membranes of both cell-types remain intact. Electronmicroscopically no evidence is found of a secretion of enzymes from the leukemia cells that destroy liver cells. Destruction of the liver cells is the consequence of the growth-pressure exerted by the dividing leukemic cells. Within the liver of the recipients the leukemic cells produce RNA-viruses. In the cytoplasm - frequently in the area of the Golgi-field - groups of immature A-particles are formed. In the virus fields the ribosomes disappear. The immature A-particles consist of two concentric electron dense shells. On the surface of the liver cells budding particles and immature C-particles develop. Extracellular mature C-particles with a homogeneous nucleoid and an irregular outer shell may be seen. The formation of viruses is found to be independent of the stage of the leukemia.
将一株NMRI品系一周龄小鼠的病毒源性、未成熟骨髓性白血病细胞腹腔注射，每只动物分别在0.2ml汉克斯平衡盐溶液中接种10⁵、10⁶或10⁷个细胞。在接种后10小时至21天的不同时间处死小鼠，并制备肝脏标本用于电子显微镜研究。接种后10小时，在肝脏的肝血窦中发现白血病细胞，30小时后在门周区域发现。白血病细胞从肝血窦迁移到狄氏间隙。白血病细胞通过连接的内皮细胞之间的细胞质指状突起穿透。内皮细胞没有溶解崩解。白血病细胞在狄氏间隙通过有丝分裂增殖，从而挤压肝细胞。两种细胞类型的细胞膜均保持完整。电子显微镜检查未发现白血病细胞分泌破坏肝细胞的酶。肝细胞的破坏是分裂的白血病细胞施加生长压力的结果。在受体的肝脏内，白血病细胞产生RNA病毒。在细胞质中——通常在高尔基体区域——形成未成熟A颗粒群。在病毒区域，核糖体消失。未成熟A颗粒由两个同心的电子致密壳组成。在肝细胞表面形成出芽颗粒和未成熟C颗粒。可以看到具有均匀核仁样结构和不规则外壳的细胞外成熟C颗粒。发现病毒的形成与白血病的阶段无关。
