Lessmann V, Gottmann K, Lux H D
Max-Planck-Institute for Psychiatry, Department of Neurophysiology, Planegg-Martinsried, FRG.
Neurosci Lett. 1992 Oct 26;146(1):13-6. doi: 10.1016/0304-3940(92)90160-9.
Fast application of L-glutamate, AMPA (alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) or kainate to cultured rat thalamic neurons revealed properties of non-NMDA (N-methyl-D-aspartate) receptors similar to those described in hippocampal neurons. The kinetics of non-NMDA receptor-mediated currents were altered by the addition of the dye Evans Blue (EB). Macroscopic desensitization was reduced and activation and deactivation kinetics were slowed. Delayed addition of EB, after desensitization of non-NMDA receptors, resulted in reactivation of desensitized receptors. Thus, both ion channel gating and entry into the desensitized state were affected. Evans blue also slowed the activation and the decay of glutamatergic miniature EPSCs (excitatory postsynaptic currents), demonstrating that receptor kinetics determine the time course of the synaptic response.
将L-谷氨酸、AMPA（α-氨基-3-羟基-5-甲基异恶唑-4-丙酸）或海人酸快速施加于培养的大鼠丘脑神经元，揭示了非NMDA（N-甲基-D-天冬氨酸）受体的特性，这些特性与海马神经元中所描述的相似。添加染料伊文思蓝（EB）改变了非NMDA受体介导电流的动力学。宏观脱敏作用减弱，激活和失活动力学减慢。在非NMDA受体脱敏后延迟添加EB，导致脱敏受体重新激活。因此，离子通道门控和进入脱敏状态均受到影响。伊文思蓝还减慢了谷氨酸能微小兴奋性突触后电流（EPSC）的激活和衰减，表明受体动力学决定了突触反应的时间进程。
