Zhou Hai-wen, Zhou Zeng-tong, Yang Wen-jun, Liu De-li, Li Qing, Hu Hong-liang, Liu Wei, Cui Lei, Cao Yi-lin
Department of Oral Medicine, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, P. R. China 200011.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2003 May;17(3):230-2.
To construct a plasmid which has a reporter gene for exploring the role of human telomerase reverse transcriptase(hTRT) in in-vitro cell cultivation.
hTRT was cut by restricted enzyme from plasmid pGRN145 and inserted to plasmid pEGFP-C1 (enhanced green fluorescent protein).
Restricted enzyme analysis and DNA sequencing showed that the sequence of the pEGFP -hTRT transgenic plasmid was correct.
The recombinant vector pEGFP-hTRT has been successfully constructed, and it can be used as a transgenic plasmid in generating immortalized cell lines.
