Zvyagilskaya R, Persson B L
A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, Moscow, 119071, Russia.
IUBMB Life. 2003 Mar;55(3):151-4. doi: 10.1080/1521654031000106681.
In this study we used a newly isolated osmo-, salt-, and alkali-tolerant Yarrowia lipolytica yeast strain, with a unique capacity to grow over a wide pH range (3.5-10.5). A procedure was elaborated to follow phosphate accumulation by Y. lipolytica cells grown at different pH values. In this paper we demonstrate that Pi-starved Y. lipolytica cells are endowed by derepressible high-affinity, high-capacity H(+)- and Na(+)-driven Pi uptake systems and that activities of these transport systems are under the dual control by the prevailing extracellular Pi concentrations and pH values.
在本研究中，我们使用了一种新分离的耐渗透压、耐盐和耐碱的解脂耶氏酵母菌株，它具有在很宽的pH范围（3.5 - 10.5）内生长的独特能力。我们精心设计了一个程序，以追踪在不同pH值下生长的解脂耶氏酵母细胞对磷酸盐的积累情况。在本文中，我们证明了缺磷的解脂耶氏酵母细胞具有可解除阻遏的高亲和力、高容量的由H⁺和Na⁺驱动的磷酸盐摄取系统，并且这些转运系统的活性受细胞外磷酸盐浓度和pH值的双重控制。
