高压氧预处理对脂多糖诱导的大鼠休克的有益作用。

Beneficial effect of hyperbaric oxygen pretreatment on lipopolysaccharide-induced shock in rats.

作者信息

Pedoto Alessia, Nandi Jyotirmoy, Yang Zhong-Jin, Wang Jingping, Bosco Gerardo, Oler Albert, Hakim Tawfic S, Camporesi Enrico M

机构信息

Research Laboratory, Department of Anaesthesiology, SUNY Upstate Medical University, Syracuse, New York 13210, USA.

出版信息

Clin Exp Pharmacol Physiol. 2003 Jul;30(7):482-8. doi: 10.1046/j.1440-1681.2003.03865.x.

DOI:10.1046/j.1440-1681.2003.03865.x

Abstract

We investigated the effect of hyperbaric oxygenation (HBO2) pretreatment on the production of exhaled nitric oxide (ENO) and the expression of lung inducible nitric oxide synthase (iNOS) by Escherichia coli lipopolysaccharide (LPS)-induced shock in an experimental rat model. 2. Rats were randomized into four groups, anaesthetized, mechanically ventilated with room air and infused with normal saline (2 mL/h) through the jugular vein for 5 h. Group 1 (NS) received only normal saline. Group 2 (HBO2-NS) was pretreated with HBO2 at 2.8 absolute atmospheres for 2 h and then received normal saline. Group 3 (LPS) received LPS, 20 mg/kg, i.v., bolus. Group 4 (HBO2-LPS) was pretreated with HBO2 for 2 h, followed by LPS. 3. Arterial blood gases, blood pressure, blood pH and ENO production were measured every 30 min. Plasma nitrite/nitrate (NOx) concentrations were assessed at the beginning (baseline) and at the end of the study. Lung myeloperoxidase (MPO) activity, iNOS expression and histological scores were measured for the evaluation of lung injury. 4. Administration of LPS was associated with decreased blood pressure and pH, increased ENO production, plasma NOx concentrations, lung iNOS expression and MPO activity. 5. Pretreatment with HBO2 significantly alleviated the LPS-induced hypotension, acidosis and decreased ENO production, plasma NOx concentrations, lung MPO activity and expression of iNOS. Hyperbaric O2 had no effect on control rats. 6. Our data show that HBO2 pretreatment has beneficial haemodynamic effects in rats with endotoxin shock. The beneficial effects of HBO2 may be partially mediated by decreased ENO production via reduced LPS-induced lung iNOS expression.

摘要

我们在实验大鼠模型中研究了高压氧（HBO₂）预处理对大肠杆菌脂多糖（LPS）诱导的休克时呼出一氧化氮（ENO）产生及肺诱导型一氧化氮合酶（iNOS）表达的影响。2. 将大鼠随机分为四组，麻醉后用室内空气进行机械通气，并通过颈静脉以2 mL/h的速度输注生理盐水5小时。第1组（NS）仅接受生理盐水。第2组（HBO₂-NS）先在2.8绝对大气压下接受HBO₂预处理2小时，然后接受生理盐水。第3组（LPS）静脉推注20 mg/kg的LPS。第4组（HBO₂-LPS）先接受HBO₂预处理2小时，随后给予LPS。3. 每30分钟测量动脉血气、血压、血液pH值和ENO产生情况。在研究开始时（基线）和结束时评估血浆亚硝酸盐/硝酸盐（NOx）浓度。测量肺髓过氧化物酶（MPO）活性、iNOS表达和组织学评分以评估肺损伤。4. 给予LPS与血压和pH值降低、ENO产生增加、血浆NOx浓度、肺iNOS表达和MPO活性增加有关。5. HBO₂预处理显著减轻了LPS诱导的低血压、酸中毒，并降低了ENO产生、血浆NOx浓度、肺MPO活性和iNOS表达。高压氧对对照大鼠无影响。6. 我们的数据表明，HBO₂预处理对内毒素休克大鼠具有有益的心血流动力学效应。HBO₂的有益作用可能部分通过降低LPS诱导的肺iNOS表达从而减少ENO产生来介导。