Chen T T, Turner D F
Department of Dentistry, National Cheng Kung University Hospital, Tainan, Taiwan, R.O.C.
J Formos Med Assoc. 1992 Aug;91(8):808-12.
In the present study, we have used the horseradish peroxidase (HRP) retrograde transport technique to map the trigeminal primary afferent and motor neurons that innervate the temporomandibular joint (TMJ) and the lateral pterygoid muscle (LPM). One to 3.0 microL of 4% wheatgerm agglutinin conjugated horseradish peroxidase (HRP-WGA) solution was introduced unilaterally into the joint cavity of twelve Sprague-Dawley rats. Injections were made from the above, following trephining of the zygomatic arch with a dental bur. Seventy-two hours after surgery, the animals were sacrificed via perfusion-fixation through the left ventricle. The TMJ, both trigeminal ganglia and brain stem were removed, sectioned serially at 50 microns, and reacted according to the tetramethyl benzidine (TMB) technique of Mesulam. Tissues were then examined at the light microscopic level. The injection site showed that the HRP-WGA filled the upper chamber of the TMJ cavity and extended anteriorly to include the LPM. HRP injected into the joint cavity was transported retrogradely to the ipsilateral trigeminal ganglion, where numerous HRP labeled neurons were found. The greatest number of cells were localized in the dorsolateral posterior portion of the ganglion. In the brain stem, numerous labeled cells representing the motor innervation of the LPM were found in a crescent-shaped array on the ventral side of the trigeminal motor nucleus. No labeled cells were found in the mesencephalic nucleus. The results show substantial trigeminal sensory innervation of the TMJ and LPM with no central projection to the mesencephalic nucleus.
在本研究中，我们运用辣根过氧化物酶（HRP）逆行运输技术来绘制支配颞下颌关节（TMJ）和翼外肌（LPM）的三叉神经初级传入神经元和运动神经元图谱。将1至3.0微升4%的小麦胚凝集素结合辣根过氧化物酶（HRP-WGA）溶液单侧注入12只Sprague-Dawley大鼠的关节腔。在使用牙钻磨除颧弓后，从上述位置进行注射。手术后72小时，通过左心室灌注固定处死动物。取出TMJ、双侧三叉神经节和脑干，以50微米的厚度连续切片，并按照Mesulam的四甲基联苯胺（TMB）技术进行反应。然后在光学显微镜水平检查组织。注射部位显示HRP-WGA充满了TMJ腔的上腔，并向前延伸至包括LPM。注入关节腔的HRP逆行运输至同侧三叉神经节，在那里发现了许多HRP标记的神经元。细胞数量最多的位于神经节的背外侧后部。在脑干中，在三叉神经运动核腹侧呈新月形排列的区域发现了许多代表LPM运动神经支配的标记细胞。在中脑核中未发现标记细胞。结果表明，TMJ和LPM有大量三叉神经感觉神经支配，且没有向中脑核的中枢投射。
